Torin 1,TOR Inhibitor Enhances Cellular Proliferation in NT-1 Tobacco Suspension Cell Cultures

• 作者: Ashutosh Sharma Elda MBeltrán-Pe?a Grisel Fierros-Romero Juan Ignacio Valiente-Banuet MElena Mellado-Rojas Reynaldo CPless
• 来源期刊: 国际实验植物学杂志(英文)
• 学科: 医学
• 关键词: Torin 1 TOR S6K PROLIFERATION NT-1 TOBACCO cells
• 年，卷（期）: 2019,（2）
• 页码范围: 131-137
• 页数: 7页
• 分类号: R73

摘要

Torin 1 is an ATP-competitive TOR inhibitor which inhibits the signaling of TOR and S6K kinase in mammals and plants.The objective of this research is to determine the effect of Torin 1 in a relatively simple and homogeneous plant system such as the NT-1 tobacco suspension cell cultures.Cultures of NT-1 cells were tested with 5,50,150 and 250 nM of Torin 1.During kinetics growth of NT-1 tobacco suspension cell cultures,150 and 250 nM Torin 1 inhibits the early growth and later enhanced the cellular proliferation during exponential growth by means of an increased expression of E2F1 and cyclin B.Furthermore,Torin 1 stimulates the growth of NT-1 cells during log phase with small shaped cell,characteristic of tobacco suspension cell cultures with high mitotic activity.