Principle and operation of Jing-well point temperatures' test is a method to diagnose and treat diseases by measuring and comparing the symmetrical Jing-well point temperature of human body 12 meridians.It is an important supplement to traditional Chinese medicine four diagnoses. The theoretical basis are that the Jing-well is closely related to Meridians and Viscera, the basic traditional Chinese medicine principle of 'governing exterior to infer interior' and the balancing phenomenon of Meridians. In this paper, the operation steps and treatment measures of Jing-well point temperatures' test are put forward, and 2 successful cases are given. At the end of this paper, the prospect of this method is discussed.