In this study, we investigated the spatial aggregation of old and incipient nests of Atta sexdens rubropilosa by fitting Poisson and Negative binomial models to nest abundance data. Our aim is to analyse the distribution of ant nests in eucalypt regrowth, Cerrado and native forest fragment. We also investigated the correlation between nest abundance and climatic factors, as well as different nest ages. When comparing nests of different ages we observed an aggregated pattern for both old and incipient nests. On the other hand, analysing the distribution of nests separately, only taking into account the different areas and respective borders, old nests exhibited an aggregated pattern and incipient nests showed a random pattern, except for native forest with ants exhibiting only an aggregated pattern. The levels of aggregation changed in response to different areas and border gradients, with more external borders showing higher aggregation than more internal borders. Temperature was the variable showing the highest correlation with nest abundance and the correlation between nests of different ages was totally depending on the different areas.