Curcumin suppresses proliferation of MCF-7 breast cancer cells by modulating gap junction signaling

• 作者: Er-Qin Dai Kai-Ming Li Lei Chen Lei Wang Mu Zhang Ning-Ning Yang Xin-Qiang Song Yu Zhang
• 来源期刊: 药物联合治疗
• 学科: 医学
• 关键词: CURCUMIN Gap JUNCTION signal pathway BREAST cancer BIOINFORMATICS analysis
• 年，卷（期）: 2019,（3）
• 页码范围: 153-164
• 页数: 12页
• 分类号: R

摘要

Curcumin, an agent isolated from turmeric, shows therapeutic potential against breast cancer. However, the mechanism underlying anticancer activity has not been fully expounded. In this research, bioinformatics was utilized to analyze molecular mechanism of curcumin and the results have been proved in breast cancer cells subsequently. In order to generate a breast cancer molecular network with which curcumin interacts, target proteins of curcumin from PubChem were combined with genes implicated in breast cancer in the NCBI within Ingenuity Pathway Analysis software. Predicted curcumin targets were verified by reverse transcription-quantitative polymerase chain reaction. Bioinformatics identified gap junction signaling and 14-3-3-mediated adherens junctions as the main canonical signaling pathways targeted by curcumin. Moreover, curcumin can substantially up-regulates the gap junction protein connexin in breast cancer cells. Our results suggest that curcumin through modulation of the gap junction signaling pathway to inhibit cell proliferation and differentiation in breast cancer cells.