Establishment and Optimization of HPLC-MS/MS for Detection of Progesterone Residue in Raw Milk

• 作者: Aifeng ZHANG Jun ZHANG Lei YAN Yuefei YAN Yuxi ZHAO Zhen ZHAGN
• 来源期刊: 农业生物技术:英文版
• 学科: 农学
• 关键词: HPLC-MS/MS FRESH and raw MILK PROGESTERONE Method ESTABLISHMENT Optimization
• 年，卷（期）: 2019,（1）
• 页码范围: 80-82
• 页数: 3页
• 分类号: S

摘要

In order to establish an HPLC-MS/MS method for detecting progesterone in raw milk,raw milk was extracted with methanol,and then determined by HPLC-MS/MS after the purification with a C 18 solid phase extraction column.Qualitative determination was performed according to retention time and selective ion abundance ratio,and quantification was performed by external standard method.The results showed that progesterone had a good linear relation in the range of 0.1-5.0 ng/ml,with a correlation coefficient of 0.999 9.The detection limit of the established method was 0.02μg/kg,and the quantification limit was 0.04μg/kg.In the standard addition range of 0.1-5.0 ng/ml,the recovery ranged from 79.6%to 105.1%,with relative standard deviation≤8.9%.Compared with the industry standard,the detection limit and the quantification limit of the established method are significantly reduced,and the sensitivity of the method is enhanced,so this method is suitable for the detection of progesterone residue in fresh milk in pastures.