Effect of Lamb Age and in vitro Culture System of Oocytes on JIVET Technology

• 作者: Chunyan LI Guanwu ZHANG Hongyu GUO Huidi LUO Huihui GUO Jun LI Peng ZHAO Shenghua ZHOU Yangyi MAO Zhiwu WANG
• 来源期刊: 农业生物技术:英文版
• 学科: 农学
• 关键词: Juvenile LAMBS Age OOCYTES In vitro culture system JIVET MATURATION RATE CLEAVAGE RATE
• 年，卷（期）: 2019,（1）
• 页码范围: 86-89
• 页数: 4页
• 分类号: S

摘要

[Objectives]This study was conducted to investigate the effects of lamb age and in vitro culture system of oocytes on the results of juvenile in vitro embryo transfer(JIVET).[Methods]Ten Dorper×small-tailed Han lambs aged 5 to 10 weeks were induced to superovulate via i.p.injection of pregnant mare s serum gonadotropin(PMSG).The oocytes were matured in basal maturation solution or modified maturation solution,which was prepared by adding 200μmol/L cysteine to the basal maturation solution.Then,the oocytes were fertilized in fertilization medium I containing 2%estrus sheep serum(ESS)or fertilization medium II containing 3 mg/ml bull serum albumin(BSA).Finally,the number of oocytes,oocyte maturation rate and cleavage rate of the lambs of different ages were determined.[Results]The average number of oocytes recovered per lamb was(111.00±16.97),(139.50±28.99),(108.50±17.68)and(42.00±11.31)for 5-,7-,8-and 10-week-old Dorper×small-tailed Han lambs,respectively.The number of oocytes obtained from 5-,7-and 8-week-old lambs was significantly higher than that from 10-week-old lambs(P<0.05),but there was no significant difference among 5-,7-and 8-week-old lambs(P >0.05).The maturation rate of oocytes cultured in modified maturation solution was 3.64%higher than that in basal maturation solution.The cleavage rate of oocytes in fertilization medium I was very significantly higher than that in fertilization medium II(P<0.01).[Conclusions]The results of JIVET can be improved by harvesting oocytes from lambs aged 5-8 weeks,adding a certain amount of cysteine into oocyte maturation solution,and a certain amount of ESS into fertilization medium.