Cellular Antioxidant Activity and Peroxidase Inhibition of Infusions from Different Aerial Parts of <i>Cassia occidentalis</i>

• 作者: Arvy Luzayana Wamba Claude Ngolo Ngolo Didi Mana Kialengila Nadege Kabamba Ngombe Pascal Kalenda T. Dibungi Pascal Tshisekedi Tshibangu Patricia Mbombo Mungisthi Paul Tshilumbu Kantola Paulin Mutwale Kapepula
• 来源期刊: 生物科学与医学(英文)
• 学科: 医学
• 关键词: CASSIA OCCIDENTALIS HRP Manalaria Monocytes HL 60 MPO NUTRACEUTICAL
• 年，卷（期）: 2019,（4）
• 页码范围: 83-94
• 页数: 12页
• 分类号: R73

摘要

Cassia occidentalis L. is widely used in the world in traditional medicine and especially in some African countries for the treatment of various diseases. The aim of this study was to report the microscopic features, the chromatographic fingerprints and the cellular antioxidant activity and the peroxidase inhibition of infusions from different parts of this plant. Microscopically, leaf can be characterized by cells of the spongy mesophyll and parenchyma numerous cluster crystals of calcium oxalate, paracytic stomata, isolated calcium oxalate cluster crystals, covering and glandular trichomes, scalariform vessels, polyedric starch granules, lignified fibers;flowers by abundant covering and glandular trichomes, spirally thickened vessels and associated parenchyma, abundant pollen grains. Seeds were characterized by pluricellular non-glandular trichomes, epidermis of the testa with underlying oil cells, parenchymatous layers of the testa, thicker-walled cells of the endosperm, pollen grain. Phytochemical analysis revealed the presence of phenolic acids, flavonoids, iridoids, tannins and terpenes. TLC fingerprints of different parts were different and characteristic. They showed the presence of glycosylated flavonoids and phenolic acids as main phytochemicals for flowers, leaves and seedpods. ABTS and DPPH assays showed that infusion extracts have the ability to scavenge free radicals connected with their IC50 values ranging from 21.43 ± 1.25 to 566.24 ± 176.7 mg·mL-1. All extracts showed a weaker capacity to scavenge DPPH radical. Aqueous extracts displayed high cellular antioxidant activity at the concentrations range of 1 - 20 μg·mL-1 using LO-12 on monocytes HL 60. Flower and leave extracts showed more efficient effects on extracellular ROS production. Phenolic compounds could be major contributors to antioxidant activity of infusions of Cassia parts. In MPO (Myeloperoxidase) direct technique, all infusion extracts exhibited a dose-dependent inhibitory effect on MPO activity in the range concentrations of 1