Effects of Macronutrients on Primary and Secondary Metabolism in Scutellaria baicalensis Georgi

• 作者: Jinhua LIU Yanjing BIAN Yongqing ZHANG Zhixia LI
• 来源期刊: 农业生物技术:英文版
• 学科: 农学
• 关键词: MACRONUTRIENTS PAL C 4H CHS Secondary METABOLITES SCUTELLARIA baicalensis
• 年，卷（期）: nyswjsywb,（2）
• 页码范围: 28-31
• 页数: 4页
• 分类号: S

摘要

[Objectives] This study was conducted to clarify the roles of macronutrients in regulating plant primary and secondary metabolism of Scutellaria baicalensis Georgi.[Methods] The contents of chlorophyll (Chl) and soluble sugar were detected with ultraviolet spectrophotometry. The activities of phenylalanine ammonia-lyase (PAL), cinnamate 4-hydroxylase (C 4H) and chalcone synthase (CHS) were determined with ultraviolet spectrophotometry. Secondary metabolites were detected by high performance liquid chromatography (HPLC).[Results] The content of chlorophyll in treatments N, P and K was increased, showing significant difference from that of the control ( P <0.05). Among them, Treatment N has the highest content of chlorophyll. Soluble sugar content in treatments N, P and K (K>N>P>CK) was increased considerably and had significant difference from that of the control ( P <0.05). Compared with the control, PAL and C 4H activity in treatments N, P and K were increased dramatically by 186.57%, 134.09%, 306.91% and 73.21%, 28.91%, 247.57%, respectively. CHS activity was also increased sharply in treatments N, P and K. Scutellarin, baicalin, wogonoside, baicalein, wogonin and oroxylin A contents in all treatments N, P and K (K>N>P) were increased, and had significant difference from that of the control ( P <0.05).[Conclusions] The results showed that macronutrients can increase leaf chlorophyll content, soluble sugar content, PAL activity, C 4H activity and secondary metabolites of S. baicalensis .