MOFzyme: FJU-21 with Intrinsic High Protease-Like Activity for Hydrolysis of Proteins

• 作者: Bin Li Danhua Ma Daomei Chen Dongqi Yang Jiaqiang Wang Jingchen Zhao Liang Jiang Lingli Li Yepeng Yang Yizhou Li
• 来源期刊: 生物科学与医学(英文)
• 学科: 化学
• 关键词: METAL-ORGANIC Frameworks (MOFs) FJU-21 PROTEASE MIMETICS REUSABILITY
• 年，卷（期）: 2019,（5）
• 页码范围: 222-230
• 页数: 9页
• 分类号: O6

摘要

In this work, metal-organic frameworks (MOFs) FJU-21 was synthesized by solvothermal method. The crystal structure of FJU-21 was characterized by XRD and BET and it was applied to the catalytic hydrolysis of bovine serum albumin. The effects of reaction pH, temperature and reaction time on the catalytic activity of FJU-21 were studied. FJU-21 were found to possess an intrinsic enzyme mimicking activity similar to that found in trypsin. The Michaelis constant (Km) of the artificial protease (0.18 × 10-3 - 0.20 × 10-3 M-1) was about 15-fold lower than that free trypsin (2.7 × 10-3 M-1) and about 3-fold lower than that of soluble Cu(II) oxacyclen (0.54 × 10-3 M-1). The Kcat of FJU-21 is 102 times higher than that of soluble Cu(II) oxacyclen catalysts and, indicating a much higher affinity of BSA for FJU-21 surface. FJU-21 could be reused for eleven times without losing in its activity.