Comparative Study on Population Ecological Distribution and Extracellular Enzyme Activities of Endophytic Fungi in <i>Artemisia annua</i>

• 作者: Bo Lian Hangao Li Yan Chen Yanna Lv Yanyan Zhao Zhen Zhang Zhihao Ji Zhuangzhuang Liu
• 来源期刊: 生物科学与医学(英文)
• 学科: 医学
• 关键词: Artemisia annua ENDOPHYTIC Fungi POPULATION Structure EXTRACELLULAR Enzymes
• 年，卷（期）: 2019,（8）
• 页码范围: 94-105
• 页数: 12页
• 分类号: R73

摘要

The endophytic fungi in different tissues of Artemisia annua was isolated and purified to explore their ecological distribution and tissue preference, and the extracellular enzyme activities of dominant endophytic fungi were determined to characterize the metabolic function of endophytic fungi. The results showed that a total of 67 endophytic fungi were obtained from Artemisia annua tissues. The number and species of endophytic fungi in different tissues were significantly different. The number, colonization rate (CR) and isolation rate (IR) of endophytic fungi in root were significantly higher than those of stem and leaf. The dominant endophytic fungi, diversity and similarity coefficient of endophytic fungi also showed significant difference among tissues. The extracellular enzyme activities of endophytic fungi in different tissues are significantly different. The enzyme activities of endophytic fungi isolated from root are significantly higher than those isolated from stem and leaf. The research results showed that the endophytic fungi in Artemisia annua had significant tissue preference, and the metabolic function of endophytic fungi showed significant difference among tissues. This will lay a foundation for further research, development and utilization of endophytic fungi, and also provide a theoretical basis for screening functional endophytic fungi in Artemisia annua.