Effect of Cytokinins on the Micropropagation of Carob (<i>Ceratonia siliqua</i>L.) through Shoot Tip Culture

• 作者: Abdelfettah Maouni Ahmed Lamarti Alain Badoc Rabah Sa?di Safaa Rahmouni Zineb Nejjar El Ansari
• 来源期刊: 美国植物学期刊(英文)
• 学科: 医学
• 关键词: CAROB Ceratonia siliqua L. CYTOKININS SHOOT Tip Culture
• 年，卷（期）: 2019,（9）
• 页码范围: 1469-1481
• 页数: 13页
• 分类号: R73

摘要

In order to determine the most suitable cytokinin for the micropropagation of carob (Ceratonia siliqua L.), the effect of four cytokinins: BAP, zeatin, kinetin and 2-iP, was tested on explants derived from young seedlings of seven days. Organogenesis is better in the presence of BAP (0.5 mg/l), while buds growth is favored by zeatin (0.5 mg/l). The combination of the most suitable cytokinin (BAP at 0.5 mg/l) with IBA improves the caulogenesis when the concentration of this latter is low (0.1 mg/l);high concentrations of IBA have an inhibitory effect on elongation and neoformation of shoots and leaves. The multiplication and growth of shoots are more favorable on WPM medium in the presence of BAP (0.5 and 1 mg/l) alone or combined with 0.5 mg/l with GA3, while rooting is mainly favored by IBA, especially at 2 mg/l.