Rescue of human corneal epithelial cells after alkaline insult using renalase derived peptide, RP-220

• 作者: Casie Phillips Hosoon Choi Luke Potts Munok Hwang Samuel Fulcher
• 来源期刊: 国际眼科杂志:英文版
• 学科: 医学
• 关键词: CORNEAL ALKALI injury renalase RP-220 human CORNEAL EPITHELIAL cells
• 年，卷（期）: 2019,（11）
• 页码范围: 1667-1673
• 页数: 7页
• 分类号: R73

摘要

AIM:To study the effect of renalase peptide,RP-220,on cell viability of human corneal epithelial cells after alkali insult.METHODS:A dose-response relationship between cell viability and exposure to NaOH solution were characterized using cultured human corneal epithelial cells.Viability of corneal epithelial cells was determined using commercially available MTT and CyQUANT?assays.RESULTS:At a concentration of 6 mmol/L,insult with NaOH leads to reduced corneal epithelial cell viability by approximately 30%.This reduced viability was prevented by treating the cells after initial insult with the 20-amino acid renalase derived peptide(RP-220).CONCLUSION:RP-220 has a pro-survival role for RP-220 following alkaline insult to corneal epithelial cells.