Study on Characteristic Fingerprint of Chemical Constituents in the Pholidota cantonensis Rolfe.

• 作者: Hua ZHU Miao ZHANG Peng LI Pingfeng LI Yuanping JIANG
• 来源期刊: 农业生物技术:英文版
• 学科: 农学
• 关键词: Pholidota cantonensis Rolfe. CHEMICAL COMPONENT FINGERPRINT HPLC
• 年，卷（期）: 2019,（5）
• 页码范围: 122-124
• 页数: 3页
• 分类号: S

摘要

[Objectives] This study was conducted to establish a characteristic fingerprinting research method for the chemical constituents of the Zhuang medicine Pholidota cantonensis Rolfe.and its characteristic fingerprint of chemical constituents,in order to identify P.cantonensis.[Methods] Ten batches of P.cantonensis from different habitats in Guangxi and out of Guangxi (Shaoguan of Guangdong Province) were determined.The materials were extracted in 80% methanol ultrasonically for 1.5 h under the material-to-liquid ratio of 2.5 g/80 ml.The HPLC determination was carried out using an Agilent 1260 HPLP instrument equipped with an Agilent5 TC-C18 (2) column at the column temperature of 30 ℃,the wavelength of 280 nm and the injection volume of 10 μl using acetonitrile-0.4% phosphoric acid as the mobile phase and chlorogenic acid as the reference peak,and the detection took 58 min.[Results] The characteristic fingerprint of the chemical constituents of P.cantonensis were obtained,and the three chromatographic peaks of gallic acid,chlorogenic acid and syringaldehyde were compared.[Conclusions] This study can provide reference for the identification of the Zhuang medicine P.cantonensis and provide guarantee for the clinical use of Chinese medicine.