The Fluorescence Immunochromatographic Strips for Natamycin and Its Application in Food Safety Detection

• 作者: Wei ZHENG Xinyue LI Yafei XU Yongfeng LIU Zhijun QIU
• 来源期刊: 农业生物技术:英文版
• 学科: 化学
• 关键词: FOOD SAFETY DETECTION NATAMYCIN FLUORESCENCE immunochromatographic ASSAY
• 年，卷（期）: 2019,（6）
• 页码范围: 104-105
• 页数: 2页
• 分类号: O62

摘要

A fluorescence immunochromatographic strip was developed in this study for natamycin detection in food. The results showed that the best amount of labeled antibody was 10 μg, for every 50 μl of fluorescent microspheres with a 2.5%(w/v) concentration. This labeled antibody was diluted for 10 times, and the diluted solution was dispensed into conjugate pad at the amount of 3 μl/cm. The concentrations of natamycin labeled BSA for test line and goat anti-mouse IgG for control line were 2.0 and 1 mg/ml, respectively, which performed best. With the best conditions, the limit of detection was 1 ng/ml, the linearity ranged from 2 to 100 ng/ml, the recovery was about 80% to 120%, and the CV was below 23%.