Effects of electroacupuncture at auricular concha area on the circadian rhythm of melatonin secretion in p-chlorophenylalanine-induced insomnia rats

• 作者: An-Ning Zhu Peng Li Shu-Qiong Huang Si-Yu Liu Wei-Wei Xu Wen-Ya Huang Xian-Jun Meng Yang Huang Zong-Bao Yang
• 来源期刊: TMR非药物治疗
• 学科: 医学
• 关键词: INSOMNIA ELECTROACUPUNCTURE of AURICULAR concha area PLASMA MELATONIN P-chlorophenylalanine
• 年，卷（期）: 2020,（1）
• 页码范围: 2-11
• 页数: 10页
• 分类号: R24

摘要

Backgroud:The electroacupuncture of auricular concha area(EA-ACR)proved to have a good regulatory effect on insomnia and the release of melatonin,which is most closely related to sleep.However,there is little research to demonstrate the relationship between EA-ACR and melatonin.The objective of present study is to observe the effects of EA-ACR on the circadian rhythm of melatonin secretion in p-chlorophenylalanine(PCPA)-induced insomnia rats.Methods:The study divided 36 male Sprague-Dawley rats randomly into three groups:12 in blank group,12 in model group,and 12 in EA-ACR group.By using PCPA intraperitoneal injection for 2 days,insomnia model was established.EA-ACR group was treated for 28 days.We observed the effects of EA-ACR on the rats'behavioral changes,open-field test and weight during the experiment.After the experiment,the rat tail and heart blood were collected,and the melatonin was detected by enzyme-linked immunosorbent assay.Results:Compared with the blank group,28–30 hours after PCPA intraperitoneal injection,the circadian rhythm of sleep and the total sleep time of model rats decreased.Compared with the model group,electrical stimulation in the ear concha region could effectively reverse the behavioral changes of rats,and increase the trend of weight growth(P=0.010),the crossing score(P=0.014),vertical motion score(P=0.015)and the level of plasma melatonin secretion(P=0.020)with the trend showing a rhythmic pattern(0–60 minutes upward,60–120 minutes downward,120–150 minutes upward).Conclusion:EA-ACR can improve the quality of life(weight),ameliorate exploring irritable and anxiety behaviors in PCPA-induced insomnia rats.The underlying effects of EA-ACR may be excited on the circadian rhythm of melatonin secretion in PCPA-induced insomnia rats.