Epigenetic regulation and mechanobiology

• 作者: Dingyi Yang Li Gao Qin Peng Shitian Li Yingxiao Wang
• 来源期刊: 生物物理学报:英文版
• 学科: 物理学
• 关键词: Epigenetic modification MECHANOBIOLOGY Live cell imaging Fluorescence resonance energy transfer(FRET)
• 年，卷（期）: 2020,（2）
• 页码范围: 33-48
• 页数: 16页
• 分类号: O57

摘要

Mechanical force regulates a variety of cellular functions through inducing modulations in nuclear chromatin structures and epigenetic landscapes(outside in).The epigenetic modifications,in turn,regulate gene expressions and affect phenotypic outcomes,including cytoskeletal organization and cellcell/cell-ECM interactions(inside out).While there have been significant advances in the understanding of mechanotransduction in the nucleus,there is still a lack of knowledge on the potential mechanisms through which mechanical cues affect epigenetic and chromatin regulations to determine genetic outcomes.This review firstly focuses on the current understanding of epigenetic regulations and then summarizes how mechanotransduction and epigenetic modification couple together to regulate molecular and cellular functions,eventually causing functional phenotype changes e.g.,diseases.Lastly,we introduce related technologies for mechanistic studies,particularly fluorescence resonance energy transfer(FRET)biosensors for the visualization of dynamic epigenetic regulations in single living cells,as well as the applications of FRET biosensors to visualize mechanotransduction events occurring in the nucleus.These studies could provide new insights into epigenetics in regulating the physiological and pathological processes in living cells under different mechanical environments.