Effect of dietary L-glutamate levels on growth,digestive and absorptive capability,and intestinal physical barrier function in Jian carp(Cyprinus carpio var.Jian)

• 作者: Juan Zhao Jun Jiang Lin Feng Pei Wu Qian Li Tian-Ran Zhang Wei-Dan Jiang Xiao-Qiu Zhou Yang Liu Ye Zhao
• 来源期刊: 动物营养:英文版
• 学科: 农学
• 关键词: L-GLUTAMATE GROWTH Digestive ability Intestinal barrier function Jian carp
• 年，卷（期）: 2020,（2）
• 页码范围: 198-209
• 页数: 12页
• 分类号: S917.4

摘要

The present study explored effects of L-glutamate(Glu)levels on growth,digestive and absorptive capability,and intestinal physical barrier functions of Jian carp(Cyprinus carpio).A total of 600 Jian carp(126.40±0.21 g)were randomly distributed into 5 groups with 3 replicates each,fed diets containing graded levels of Glu(53.4[control],57.2,60.6,68.4,and 83.4 g/kg)for 63 d.Results showed compared with control diet,feed intake and percent weight gain(PWG)in fish fed 83.4 g of Glu/kg diet were increased and feed conversion ratio in fish fed 68.4 g of Glu/kg diet was decreased(P<0.05).Similarly,body crude protein and lipid contents in fish fed 68.4 g of Glu/kg diet were higher(P<0.05).The activities of trypsin and chymotrypsin in the hepatopancreas and intestine,and amylase,alkaline phosphatase(AKP),Na~+,K~+-ATPase(NKA),and creatine kinase(CK)in intestine were higher in fish fed 68.4 g of Glu/kg diet(P<0.05).Dietary Glu(57.2 to 83.4 g/kg diet)decreased malondialdehyde(MDA)and protein carbonyl(PCO)contents in the intestine(P<0.05).The activities of catalase(CAT),glutathione peroxidase(GPx),and glutathione S-transferase(GST)in the hepatopancreas and intestine were higher in fish fed 60.6 and 68.4 g of Glu/kg diets(P<0.05).Intestinal the glutathione reductase(GR)activity and glutathione(GSH)content in fish fed 60.6,68.4,and 83.4 g of Glu/kg diet were increased(P<0.05).The GPx1 a,GST,and nuclear factor erythroid 2-related factor 2(Nrf2)mRNA expressions in the intestine were up-regulated in fish fed 60.6 and 68.4 g of Glu/kg diet(P<0.05).The zonula occludens protein-1(ZO-1),occludin1,and claudin3 mRNA expressions were also up-regulated in fish fed 83.4 g of Glu/kg diet(P<0.05).Fish fed 68.4 g of Glu/kg diet had higher levels of claudin 2,claudin7,and protein kinase C(PKC)mRNA(P<0.05).These results indicated that Glu improved frsh growth,digestive and absorptive ability,and intestinal physical barrier functions.Based on the quadratic regression analysis of PWG,and MDA of the hepatopancreas and intestine,the optimal dietar