Specificity of Various Mitochondrial DNA (<i>mt</i>DNA), <i>ND5</i>, <i>D-Loop</i>, and <i>Cty-b</i>DNA Primers in Detecting Pig (<i>Sus scrofa</i>) DNA Fragments

• 作者: Estri Laras Arumingtyas Joni Kusnadi Noval Audi Ashari
• 来源期刊: 美国分子生物学期刊(英文)
• 学科: 数学
• 关键词: Cyt-b D-Loop mt-DNA ND5 Pigs Pork SPECIFICITY
• 年，卷（期）: 2020,（3）
• 页码范围: 141-147
• 页数: 7页
• 分类号: O17

摘要

<div style="text-align:justify;"> Polymerase Chain Reaction (PCR) is an accurate, simple and fast analytical method. This technique is widely used in the identification of meat adulteration and meat-based processed food products. Three Mitochondrial DNA (mt-DNA) primers NADH Dehydrogenase sub unit 5 (<em>ND5</em>), <em>D-Loop</em>, and Cytochrome b (<em>Cyt-b</em>) were tested for their specificity in detecting of pig (<em>Sus scrofa</em>) DNA fragments. DNA genome from 6 meat samples (pork, beef, goat, lamb, and chicken) was amplified by PCR technique using three pairs of primers (<em>ND5, D-Loop</em><em>, </em>and <em>Cyt-b</em>) and sequenced. The results of amplification using the three primers produced specific DNA bands with the lengths of 232 bp, 951 bp, and 404 bp, respectively. Comparison results with<em> ND5, D-Loop,</em> and <em>Cyt-b</em> gene sequences resulted in similarity values of 100%, 97%, and 99%, respectively. These showed that the mt-DNA primers of <em>ND5, D-Loop</em>, and<em> Cyt-b </em>genes can be recommended as specific primers in detecting pig (<em>Sus scrofa</em>) DNA fragments. </div>