The Amplification and Application of Ribosomal RNA (rDNA) Gene Sequences of <i>Blidingia minima</i>(Chlorophyta, Blidingia)

• 作者: Songdong Shen
• 来源期刊: 海洋科学期刊(英文)
• 学科: 数学
• 关键词: Blidingia minima RDNA IGS (Intergenic Spacer) Intraspecies Relationships
• 年，卷（期）: 2020,（3）
• 页码范围: 177-190
• 页数: 14页
• 分类号: O17

摘要

<span style="font-family:Verdana;">The sequence of the ribosomal RNA gene (rDNA) plays an important role in species identification and phylogenetic analysis. However, the only published </span><span><span style="font-family:Verdana;">full-length sequence of a ribosomal gene of green algae is that of </span><i><span style="font-family:Verdana;">Ulva mutabilis</span></i><span style="font-family:Verdana;">.</span></span><span style="font-family:Verdana;"> In this study, we </span><a name="_Hlk17805857"></a><span style="font-family:Verdana;">amplified the full-length sequence of each ribosomal gene unit of the ribosomal gene of </span><i><span style="font-family:Verdana;">Blidingia minima</span></i><span style="font-family:Verdana;">. The full-length sequence of the ribosomal gene in </span><i><span style="font-family:Verdana;">Blidingia minima</span></i><span style="font-family:Verdana;"> is 8676 bp, including the 1759 bp 18S rDNA, 576 bp internal transcribed spacer (ITS) + 5.8S rDNA, 3282 bp 28S </span><span style="font-family:Verdana;">rDNA, and 3059 bp intergenic spacer (IGS) region. We then carried out a series</span><span style="font-family:Verdana;"> of genetic analyses based on the ITS and IGS sequences, to verify whether IGS </span><span><span style="font-family:Verdana;">sequences are useful for studying the genetic diversity of green algae from different locations. We amplified the IGS sequences of </span><i><span style="font-family:Verdana;">Blidingia minima</span></i><span style="font-family:Verdana;"> from 10 different locations in the Yellow Sea. Multiple alignments of the IGS sequences </span></span><span style="font-family:Verdana;">of samples from these 10 different sites revealed varying degrees of base </span><span><span style="font-family:Verdana;">differences, and comparative analysis of the ITS sequences revealed that our amplified species was classified as </span><i><span style="font-family:Verdana;">Blidingia minima</span></i><span style="font-family:Verdana;"> and distinct from other green a