An Investigation of Polymorphism in <i>SMO</i>and <i>LMF</i>1 Genes and Their Association with Body Size in White Fulani and Muturu Cattle Breeds

• 作者: Ibrahim Shu’aibu Matthew J. Hegarty Ridwan O. Ahmed Semiu F. Bello
• 来源期刊: 生命科学与技术进展(英文)
• 学科: 数学
• 关键词: SMOOTHENED Lipase Maturation Factor 1 Body Measurement Traits White Fulani Muturu POLYMORPHISM
• 年，卷（期）: 2020,（7）
• 页码范围: 319-344
• 页数: 26页
• 分类号: O17

摘要

Cattle production is integral to the people of Africa and her economy. To <span style="font-family:Verdana;">improve cattle productivity, there is a need to inculcate molecular mark</span><span style="font-family:Verdana;">er-assisted selection into current breeding practices. In this study, we investigated, in </span><span style="font-family:Verdana;">White Fulani and Muturu cattle breeds, polymorphism of two candidate</span><span style="font-family:Verdana;"> genes (Smoothened—</span><i><span style="font-family:Verdana;">SMO</span></i><span style="font-family:Verdana;"> and Lipase Maturation Factor 1—</span><i><span style="font-family:Verdana;">LMF</span></i><span style="font-family:Verdana;">1</span><span style="font-family:Verdana;">) that have been reported to influence body size in cattle. The </span><i><span style="font-family:Verdana;">SMO</span></i><span style="font-family:Verdana;"> gene encodes for the SMO </span><span style="font-family:Verdana;">(smoothened) receptor protein;a membrane signal transducer that plays a</span><span style="font-family:Verdana;"> role in the control of bone formation and adipogenesis. </span><i><span style="font-family:Verdana;">LMF</span></i><span style="font-family:Verdana;">1 </span><span style="font-family:Verdana;">encodes for the </span><span style="font-family:Verdana;">LMF1 protein which regulates post-translational maturation of vascular li</span><span style="font-family:Verdana;">pases. Desired regions of the genes were amplified and genotyped by direct </span><span style="font-family:Verdana;">DNA sequencing or Polymerase chain reaction—Restriction Fragment</span><span style="font-family:Verdana;"> Length </span><span style="font-family:Verdana;">Polymorphism. For association study, linear body measurements traits</span><span style="font-family:Verdana;"> (BMTs) that can be used to predict body size were measured on each animal and values of BMTs measured were compared between both breeds. Very significant (P < 0.001) differences, in favour of White Fulani