Evaluation of the Essential Oil of <i>Citrus paradisi</i>as an Alternative Treatment against <i>Candida albicans</i>

• 作者: Abelardo Chávez Montes Ameyalli J. Martinez Delgado Juan G. Báez González Laura Elena Villarreal García Osvelia E. Rodríguez Luis Rosa María Sánchez Casas Sonia M. López Villarreal Uziel Castillo Velázquez
• 来源期刊: 口腔学期刊(英文)
• 学科: 化学
• 关键词: Citrus Candida CANDIDIASIS Essential Oil Herbal Medicine
• 年，卷（期）: 2020,（9）
• 页码范围: 258-270
• 页数: 13页
• 分类号: O62

摘要

<strong>Introduction:</strong> The World Health Organization establishes that 80% of the world’s population uses traditional medicine for their primary care, because they contain compounds responsible for their properties. <strong>Objective:</strong> To evaluate the antifungal effect of the essential oil of <i>Citrus paradisi<span style="white-space:nowrap;"></span></i> against <i>C. albicans<span style="white-space:nowrap;"></span></i> and the cytotoxic effect in three cell lines <em>in vitro</em>. <strong>Methods:</strong> The phytochemical characterization of the oil was carried out by chemical methods and Gas chromatography (GC-MS) and the antifungal effect against <i>C. albicans<span style="white-space:nowrap;"></span></i> (ATCC 90029) was evaluated by the Kirby-Bauer method, which evaluated concentrations of 0.75 - 20 μg/mL and compared with nystatin 100,000 Ul/mL as a positive control. The percentage of the relative inhibitory effect was calculated. The minimum inhibitory concentration (MIC) was determined at 24 hours. Moreover, the cytotoxic effect on <em>C. albicans</em> and cell lines was determined by the colorimetric MTT tetrazolium assay. Finally, the antifungal effect against <em>Candida</em> strains isolated from clinical samples was evaluated at a concentration of 20 μg/mL. <strong>Results:</strong> The essential oil showed an antifungal effect with a percentage of inhibition of 123%. The MIC was 2.5 μg/mL, and the cytotoxicity index was 5.44 μg/mL for <em>C. albicans</em>. The IC50 values were 21.060, 9.482 and 4.176 μg/mL for Vero E6, J774A.1 and MDBK cells respectively. <strong>Conclusion:</strong> These results show the use of <em>C. paradisi</em> essential oil as an alternative treatment in oral antifungal therapy, it is beneficial due to its antifungal effect and its low toxicity on cell cultures.