Advance of Multiplex PCR in Rapid Detecting Transgenic Soybean Oil

• 作者: Siya Yin Yi Liu
• 来源期刊: 美国植物学期刊(英文)
• 学科: 经济
• 关键词: TRANSGENIC SOYBEAN OIL DNA EXTRACTION Method Multiplex PCR
• 年，卷（期）: 2020,（3）
• 页码范围: 382-392
• 页数: 11页
• 分类号: F42

摘要

Transgenic food safety is a high-profile public health issue in worldwide, especially transgenic soybean (Glycine max L.) oil. To rapidly and effectively detect transgenic components of soybean oil, in the present study, we isolated DNA from transgenic soybean oil by modified method, and employed the multiplex PCR method to identify targeted genes, including CaMV35S promoter, Nos terminator, NPTII, CP4-EPSPS and endogenous gene Lectin. The research aims to build a method which is accurate, rapid and reliable for detection of genetically modified soybeans oil. The targeted gene including DNA was successfully established by the improved method, and then amplified by PCR. Five genes are simultaneously specifically detected. Commercial soybean, genetically modified soy bean and oil were detected with the Multiplex PCR. The improved method of DNA extraction was rapid and accurate to extract high quality total DNA which was amplified by PCR. The method could eliminate the PCR inhibitor. A way of detecting the genetically modified soybean and Oil was set up in this study.