LINC00511 promotes gastric cancer cell growth by acting as a ceRNA

• 作者: Chong-Bing Sun Hong-Xia Zhang Hong-Yi Wang Meng-Chun Wang Xiao-Gang Leng Xiao-Qing Han Yong-Ning Liu You-Feng Gu
• 来源期刊: 世界胃肠肿瘤学杂志:英文版(电子版)
• 学科: 医学
• 关键词: LINC00511 miR-124-3p PDK4 LONG noncoding RNAS GASTRIC cancer
• 年，卷（期）: 2020,（4）
• 页码范围: 394-404
• 页数: 11页
• 分类号: R735.2

摘要

BACKGROUND Gastric cancer(GC)is one of the most aggressive malignancies,with a high incidence and poor prognosis worldwide.Recently,accumulating evidence has illustrated that long noncoding RNAs(lnc RNAs)play pivotal roles in many cancers.It has been reported that LINC00511 contributes to tumorigenesis in various diseases.However,the role of LINC00511 in GC cell growth remains mostly unknown.AIM To determine whether the lnc RNA LINC00511 exerted its carcinogenic function in GC via the miR-124-3 p/PDK4 axis.METHODS Cell culture and transfection,RNA extraction and quantitative real-time PCR,CCK-8 assay,Colony formation assay,Luciferase reporter assay,RIP assay,RNA pull-down assay,and Western blot analysis were used to show expression and mechanisms of LINC00511 in GC progression and apoptosis.Rescue assays were performed to verify the relationships among LINC00511,miR-124-3 p and PDK4 further.RESULTS The expression of LINC00511 was remarkably upregulated in GC cells compared to that in corresponding normal cell lines.Compared to the controls,cell proliferation was inhibited,and cell apoptosis was increased upon LINC00511knockdown,demonstrating that LINC00511 influenced GC cell growth.An exploration of the molecular mechanism revealed that LINC00511 functioned as a molecular sponge of miR-124-3 p and that PDK4 was a downstream target of miR-124-3 p in GC.Rescue assays showed that the overexpression of PDK4 could partly restore the inhibitory function of si-LINC00511 in GC.CONCLUSION These data demonstrate that LINC00511 promotes gastric cancer cell growth by acting as a ce RNA to regulate the miR-124-3 p/PDK4 axis,which may be a promising therapeutic target for GC.