Antimicrobial Activity of a Formulation of <i>Libidibia ferrea</i>L. against Microorganisms of the Dental Biofilm

• 作者: Carina Toda Fábio Correia Sampaio Gisely Naura Venâncio Maria Fulgência Costa Lima Bandeira Nikeila Chacon de Oliveira Conde Raquel de Oliveira Marreiro Tatiane Pereira de Souza Wanderleia Monteiro de Souza
• 来源期刊: 微生物学(英文)
• 学科: 医学
• 关键词: MOUTHWASH Juca Natural Products
• 年，卷（期）: 2020,（9）
• 页码范围: 434-442
• 页数: 9页
• 分类号: R78

摘要

This study aimed to evaluate the antimicrobial activity of the extract of <i>Libidibia ferrea</i> L. (228.022-INPA) and a mouthwash formulation against microorganisms of the dental biofilm. The extract of <i>Libidibia ferrea</i> L. was prepared and formulated with an alcohol-free mouthwash. The Minimum Inhibitory Concentration—MIC was determined by microdilution and cell viability was evaluated through fluorescence. The MICs for the extract were 4.375 μg/mL (<i>Streptococcus mutans</i>), 3.750 μg/mL (<i>Streptococcus oralis</i>), 4.375 μg/mL (<i>Lactobacillus casei</i>). In contrast, for the mouthwash, the MICs were 6.000 μg/mL (bacteriostatic against <i>Streptococcus mutans</i> and bactericidal against <i>Streptococcus oralis</i>). In 1.250 μg/mL of the extract, the number of viable cells measured by fluorescence tests was 16.47% and 14.12% for <i>Streptococcus mutans</i> and <i>Lactobacillus casei</i>, respectively. For the mouthwash (1.000 μg/mL), 20.60% of viable cells for <i>Streptococcus mutans</i>, and 9.60% for <i>Lactobacillus casei</i> were observed. It can be concluded that the extract and its mouthwash containig <i>Libidibia ferrea</i> L. showed antibacterial activity against microorganisms from the dental biofilm.