Multifocal photoacoustic microscopy using a single-element ultrasonic transducer through an ergodic relay

• 作者: Yang Li Terence T.W.Wong Junhui Shi Hsun-Chia Hsu Lihong V.Wang
• 来源期刊: 光：科学与应用（英文版）
• 年，卷（期）: 2020,（5）
• 所属期刊栏目: Letters
• 页码范围: 1299-1305
• 页数: 7页
• 语种: 英文

摘要

Optical-resolution photoacoustic microscopy (OR-PAM) has demonstrated high-spatial-resolution imaging of optical absorption in biological tissue. To date, most OR-PAM systems rely on mechanical scanning with confocally aligned optical excitation and ultrasonic detection, limiting the wide-field imaging speed of these systems. Although several multifocal OR-PA (MFOR-PA) systems have attempted to address this limitation, they are hindered by the complex design in a constrained physical space. Here, we present a two-dimensional (2D) MFOR-PAM system that utilizes a 2D microlens array and an acoustic ergodic relay. Using a single-element ultrasonic transducer, this system can detect PA signals generated from 400 optical foci in parallel and then raster scan the optical foci patterns to form an MFOR-PAM image. This system improves the imaging resolution of an acoustic ergodic relay system from 220 to 13μm and enables 400-folds shorter scanning time than that of a conventional OR-PAM system at the sameresolution and laser repetition rate. We demonstrated the imaging ability of the system with both in vitro and in vivo experiments.