Detection of <i>Legionellae pneumophila</i>in Waters by Culture and Polymerase Chain Reaction

• 作者: Omar Bashir Ahmed
• 来源期刊: 微生物学(英文)
• 学科: 医学
• 关键词: Legionella pneumophila PCR BCYNE Agar GPVC Agar Water Samples
• 年，卷（期）: 2020,（9）
• 页码范围: 456-462
• 页数: 7页
• 分类号: R73

摘要

<i>Legionella pneumophila</i> (<i>L. pneumophila</i>) is the most common causative agents for all outbreaks of Legionnaires’ disease. Prevention and control of Legionellosis requires surveying and monitoring of <i>Legionella</i> in the environment using conventional and modern technologies. The present study aims to compare detection of <i>L. pneumophila</i> in water samples using both culture and PCR techniques. A pre-enriched contaminated water sample was split into 13 subsamples. Culture and PCR tests were done from the subsamples after different intervals. The results showed a positive PCR result for <i>L. pneumophila</i> after 8 h of incubation. Also, <i>L. pneumophila</i> was detected by culture on non-selective BCYNE agar and selective GPVC agar after 5 and 6 days of incubation respectively. There was no significant difference between the non-selective BCYE- and the selective GVPC method. The PCR procedure was found more sensitive and differed significantly from the conventional selective GVPC method in isolation of <i>L. pneumophila</i> from water samples. It was concluded that pre-enrichment incubation allows the detection of <i>L. pneumophila</i> by PCR within a maximum of 12 h from the collection of water samples.