Kinetics of <i>Candida albicans</i>and <i>Staphylococcus aureus</i>Biofilm Initiation on Herpes Simplex Virus (HSV-1 and HSV-2) Infected Cells

• 作者: Amber Kaminski Balbina J. Plotkin Brent Ito Ira M. Sigar Jessica Kreamer Joan Kacmar
• 来源期刊: 微生物学(英文)
• 学科: 医学
• 关键词: Polymicrobic MICROBIOME BIOFILM ADHERENCE Herpes Simplex Virus Staphylococcus aureus Candida albicans
• 年，卷（期）: 2020,（11）
• 页码范围: 583-598
• 页数: 16页
• 分类号: R37

摘要

This study examines the kinetics of <i>S. aureus</i> and <i>C. albicans</i> adherence as it relates to HSV replication and corresponding dynamic display of shared receptors. HeLa cells infected for various times with HSV-1 gL86 or HSV-2 333gJ-(MOI 50) were incubated with <i>S. aureus</i> ATCC 25923 or <i>C. albicans</i> yeast and CFU measured. Over time, <i>S. aureus</i> adherence to HSV-1 infected cells was relatively stable for 45 min then decreased to 0.8 of virus-free control, before cycling at 15-to-30 min intervals. In contrast, staphylococcal adherence to HSV-2 infected cells proceeded at a more gradual rate, increasing to control levels at ~105 min before decreasing to a nadir at 165 min. Yeast adherence to HSV-1 infected cells remained relatively unchanged for the first 75 min then increased 2-fold before returning to its original level. This pattern is repeated over the next 90 min. While a similar pattern with <i>C. albicans</i> and HSV-2 was measured, it occurred more rapidly. Our model shows that while the interaction of both HSV-1 and HSV-2 with <i>S. aureus</i> is both dynamic and inhibitory, <i>C. albicans</i> interaction with HSV-2 is more permissive than HSV-1. However, the interaction of both microbes with HSV-infected cells in this model system appears to be independent of α5B1, CD36 and HSP60 viral-regulated receptor expression. These findings indicate that microbiome interactions across taxonomic kingdoms are more complex than previously thought.