Three Tiaobu Feishen formulae reduces cigarette smoke-induced inflammation in human airway epithelial cells

• 作者: Chen Yulong Dong Haoran Feng Suxiang Li Jiansheng Liu Xuefang Qin Yanqin Wu Yaosong Yin Sugai Zhao Peng Zheng Wanchun
• 来源期刊: 中医杂志（英文版）
• 年，卷（期）: 2020,（3）
• 所属期刊栏目: Research Articles
• 页码范围: 386-392
• 页数: 7页
• 语种: 英文

摘要

OBJECTIVE: To investigate the therapeutic efficacy of Tiaobu Feishen formulae (TBFS) on cigarette smoke-induced inflammation in vitro using lipopolysaccharide (LPS)-induced and cigarette smoke extract (CSE)-induced NCI-H292 cells.METHODS: We evaluated the inhibitory effects of Bufei Jianpi formula (BJF),Bufei Yishen formula (BYF),and Yiqi Zishen formula (YZF) on the expressions of inflammatory cytokines including tumor necrosis factor (TNF)-α and interleukin (IL)-8,matrix metalloproteinase (MMP)-9,tissue inhibitor of matrix metalloprotease (TIMP)-1,and superoxide dismutase (SOD) in H292 cells stimulated with LPS or CSE.Their related transcription factors and signaling pathways were also analyzed.RESULTS: BJF,BYF,and YZF significantly inhibited the LPS-or CSE-induced expressions of TNF-α,IL-8,MMP-9,TIMP-1,and SOD in H292 cells,and suppressed the activation of transcription factors including nuclear transcription factor (NF)-KB,activator protein (AP)-1,and signal transducers and activators of transcription (STAT) 3 and their corresponding pathways,including NF-KB,mitogen-activated protein kinase (MAPK),STAT3,and peroxisome proliferator-activated receptor (PPAR).CONCLUSION: BJF,BYF,and YZF effectively suppressed inflammatory responses,protease-antiprotease imbalance,and oxidative stress induced by LPS and CSE,an effect that was closely associated with the inhibition of the NF-KB,MAPK,STAT3,and PPAR pathways.