Hepatitis A Virus PCR Analysis and <i>E. coli</i>Detection in Oysters at Oualidia Lagoon and Their Correlation

• 作者: Fatiha El Mellouli Hasnae Zekhnini Nabil Abouchoaib Naima El Moqri Najwa Hassou Samira Etahiri
• 来源期刊: 食品与营养科学(英文)
• 学科: 数学
• 关键词: Hepatitis A Virus E. coli Bivalves SHELLFISH RT-PCR Oualidia Lagoon
• 年，卷（期）: 2020,（7）
• 页码范围: 684-694
• 页数: 11页
• 分类号: O17

摘要

The present study aims to evaluate hepatitis A virus (HAV) prevalence and faecal contamination indicators <span style="font-family:Verdana;"><i></i></span><i><i><span style="font-family:Verdana;">Escherichia coli</span></i><i><span style="font-family:Verdana;"></span></i></i><span style="font-family:Verdana;"> (</span><span style="font-family:Verdana;"><i></i></span><i><i><span style="font-family:Verdana;">E. coli</span></i><i><span style="font-family:Verdana;"></span></i></i><span style="font-family:Verdana;">) in oysters from Oualidia lagoon (Moroccan Atlantic coast) and to study the correlation between the two parameters. The survey was carried out on 87 samples of oysters (</span><span style="font-family:Verdana;"><i></i></span><i><i><span style="font-family:Verdana;">Crassostrea gigas</span></i><i><span style="font-family:Verdana;"></span></i></i><span style="font-family:Verdana;">) collected monthly between November 2015 and February 2017 from three sites corresponding to different oyster farms in the lagoon. Sanitary status of bivalve molluscs was assessed by </span><span style="font-family:Verdana;"><i></i></span><i><i><span style="font-family:Verdana;">E. coli</span></i><i><span style="font-family:Verdana;"></span></i></i><span style="font-family:Verdana;"> enumeration using ISO 16649-3. Detection of hepatitis A virus, was carried out by real-time reverse transcriptase polymerase chain reaction (rRT-PCR) according to ISO 15216-2 method. The prevalence of samples for which </span><span style="font-family:Verdana;"><i></i></span><i><i><span style="font-family:Verdana;">E. coli</span></i><i><span style="font-family:Verdana;"></span></i></i><span style="font-family:Verdana;"> contamination exceeds the threshold of 230 </span><span style="font-family:Verdana;"><i></i></span><i><i><span style="font-family:Verdana;">E. coli</span></i><i><span style="font-family:Verdana;"></span></i></i><span style="font-family:Verdana;">/100g of flesh and intravalvular fluid (FIF) is 43%. HAV RNA was detected in 2% of the sampl