Antioxidant, Anti-Inflammatory Efficacy and HPLC Analysis of <i>Annona muricata</i>Leaves Extracts from Republic of Benin

• 作者: Baba-Moussa Lamine Chabi Nicodème Dah-Nouvlessounon Durand Dougnon Victorien Kohonou A. Christian Kohonou Arnaud Lehmane Halfane N’Tcha Christine Nounagnon Martial Pacôme Noumavo Sina Haziz Sognigbe Basile
• 来源期刊: 美国植物学期刊(英文)
• 学科: 医学
• 关键词: A. muricata Secondary Metabolites Extracts Screening Biological Activities BENIN
• 年，卷（期）: 2020,（6）
• 页码范围: 803-818
• 页数: 16页
• 分类号: R28

摘要

<i><span style="font-family:Verdana;">Annona</span></i><span style="font-family:""> <i><span style="font-family:Verdana;">muricata</span></i><span style="font-family:Verdana;"> L. (Soursop or Graviola) is a naturally occurring plant seen in Southern part of Africa, traditionally used in Benin to treat various diseases. The present study aimed to investigate phytochemical composition and antioxidant, anti-inflammatory activities of </span><i><span style="font-family:Verdana;">A</span></i><span style="font-family:Verdana;">. </span><i><span style="font-family:Verdana;">muricata</span></i><span style="font-family:Verdana;"> leaves extracts. The secondary metabolites of ethanolic and hemi-ethanolic extracts were analysed by HPLC method. The DPPH and FRAP methods were used to evaluate the antioxidant activity. Inhibition of albumin denaturation method was used to evaluate anti-inflammatory activity of the tested extracts of which larval cytotoxicity was st</span><span><span style="font-family:Verdana;">udied. The major identified compounds were gallic acid, chlorogenic acid, cafeic acid, tannic acid, ferrulic acid, Rutin. Ascorbic acid exhibited the highest inhibition percentage (83.33% ± 0.50%) of DPPH radical with the lowest IC</span>_{<span style="font-family:Verdana;">50</span>}<span style="font-family:Verdana;"> (45.1 ± 0.28 μg/ml). The inhibition of the ferric ion Fe</span>^{<span style="font-family:Verdana;">3+</span>}<span style="font-family:Verdana;"> varied (</span><i><span style="font-family:Verdana;">p</span></i><span style="font-family:Verdana;"> = 0.0013) according to the extracts type. IC</span>_{<span style="font-family:Verdana;">50</span>}<span style="font-family:Verdana;"> values of ferric ion inhibition range from 119.5 ± 3.10 to 250.8 ± 2.13 μg/ml respectively for </span><i><span style="font-family:Verdana;">A</span></i><span style="font-family:Verdana;">. </span><i><span style="font-family:Verdana;">muricata</span></i><span style="font-family:Verdana;"> leaves ethanol