Protective Effect of <i>Lepedium sativum</i>against 2, 4-Dichlorophenoxyacetic Acid Hepatotoxicity: An Electron Microscopic Study

• 作者: Awatef Ali Gehan A. Raouf Tahani H. Dakhakhni
• 来源期刊: 应用科学(英文)
• 学科: 数学
• 关键词: HEPATOPROTECTIVE Toxicity Lepedium sativum 2 4-Dichlorophenoxyacetic Acid
• 年，卷（期）: 2020,（12）
• 页码范围: 825-833
• 页数: 9页
• 分类号: O17

摘要

<div style="text-align:justify;"> <span style="font-family:Verdana;">Medicinal plants have been used to treat many diseases with fewer side effects compared to clinical medicines. The need for treatment by medicinal plants has been increasing in response to the accumulation of environmental pollutants. The herbicide 2, 4-Dichlorophenoxyacetic acid has been considered as fast spreadable environmental pollutant, which may cause many disorders in animals and possibly in humans. The aim of this study is to assess the protective and/or curative effect of </span><i><span style="font-family:Verdana;">Lepidium sativum</span></i><span style="font-family:Verdana;"> against hepatotoxicity induced by 2, 4-D herbicide in rats. 25 Albino Wistar rats were grouped into 4 groups: a control group, the curative group treated for 4 weeks with LS water extract after a single dose 2, 4-D LD</span>_{<span style="font-family:Verdana;">50</span>}<span style="font-family:Verdana;"> administration, protective group which was treated for 4 weeks with LS water extract prior to the 2, 4-D LD</span>_{<span style="font-family:Verdana;">50</span>}<span style="font-family:Verdana;"> administration, and LS only group that was given the LS water extract as a positive control. Using transmission electron microscope, the histological features showed that curative group hepatocytes still suffered from focal necrosis and vacuolation of hepatocytes as a result of 2, 4-D toxic effect whereas in the protective group hepatocytes were less accentuated expressed as less frequent necrotic area, and still large mitochondria were present with numerous undissolved lipid droplets and a small amount of rough endoplasmic reticulum. For the LS only group, nearly normal cells with centric nucleus, normal small mitochondria were at a normal nucleus-cytoplasmic ratio, but a few lipid droplets were noticed. This study proved that curative group has an advantage over the protective group that could be presented in the nucleus with narrow nuclear pores and