Drug resistance gene expression and chemotherapy sensitivity detection in Chinese women with different molecular subtypes of breast cancer

• 作者: Bo Sun Hailian Zhang Jing Zhao Juntian Liu Meng Wang Nan Wu Shichao Zhang Ting Lei
• 来源期刊: 癌症生物学与医学（英文版）
• 年，卷（期）: 2020,（4）
• 所属期刊栏目: ORIGINAL ARTICLE
• 页码范围: 1014-1025
• 页数: 12页
• 语种: 英文
• DOI: 10.20892/j.issn.2095-3941.2020.0157

摘要

Objective:The aim of the study was to identify specific chemosensitivity drugs for various molecular subtypes of breast tumors in Chinese women, by detecting the expression of drug resistance genes and by using the drug sensitivity test on different molecular subtypes of breast cancers. Methods: The expression of drug resistance genes includingTopo Ⅱ, GST-π, P-gp, LRP,and CD133were detected with immunohistochemistry in a tissue microarray. Drug sensitivity tests included those for paclitaxel, epirubicin, carboplatin, vinorelbine, and fluorouracil and were conducted on primary cancer tissue cells and cell lines, including the T47D, BT-474, and MDA-MB-231 cells and human breast cancer xenografts in nude mice. Results: The different drug resistant genesTopo Ⅱ, GST-π, P-gp,and LRP were differentially expressed among different molecular subtypes of breast cancers (P< 0.05). Positive expression of CD133 was highest in basal-like breast cancer (P< 0.05). Kaplan-Meier survival analysis showed that positive expressions of Topo Ⅱ and CD133 both correlated with shorter disease-free survival (DFS) (P< 0.05) and overall survival (P< 0.05), and positive expression of LRP correlated only with shorter DFS (P< 0.05). BT-474 showed chemosensitivity to paclitaxel and epirubicin, while MDA-MB-231 showed chemosensitivities to paclitaxel, epirubicin, carboplatin, and fluorouracil (T/C≤ 50％). The basal-like and HER2+ breast cancer primary cells showed chemosensitivities to paclitaxel and epirubicin with significant differences compared with luminal breast cancer primary cells (P< 0.05). Conclusions: The differential expression of drug resistance genes and the differential chemosensitivities of drugs in different molecular subtype of breast cancers suggested that individual treatment should be given for each type of breast cancer.

作者信息

序号	姓名	单位	发文数	被引次数	H指数	G指数
1	Jing Zhao		1	0	0.0	0.0
2	Hailian Zhang		1	0	0.0	0.0
3	Ting Lei		2	0	0.0	0.0
4	Juntian Liu		1	0	0.0	0.0
5	Shichao Zhang		1	0	0.0	0.0
6	Nan Wu		1	0	0.0	0.0
7	Bo Sun		1	0	0.0	0.0
8	Meng Wang		1	0	0.0	0.0