Micropropagation of the Moroccan Endemic Plant <i>Thymus broussonetii</i>Boiss. with Aromatic-Medicinal Value and Conservation Concern

• 作者: Ahmed Lamarti Aïcha El Oualkadi Alain Badoc Houda Tahiri Ibtissam Boussaoudi Patrick Martin Rajae Benkaddour Zineb Nejjar El Ansari
• 来源期刊: 美国植物学期刊(英文)
• 学科: 数学
• 关键词: Thymus broussonetii MICROPROPAGATION Shoot Tip Nodes Macronutrients Cytokinins Polyamines AUXINS Prototypes CONSERVATION
• 年，卷（期）: 2020,（6）
• 页码范围: 913-938
• 页数: 26页
• 分类号: O17

摘要

Micropropagation from shoot tips and nodal segments was carried out for the conservation and domestication of spontaneous Moroccan thyme, <i><span style="font-family:Verdana;">Thymus broussonetii</span></i><i> </i><span style="font-family:Verdana;">Boiss. subsp. </span><i><span style="font-family:Verdana;">broussonetii</span></i><span style="font-family:Verdana;"> (endemic threatened). The mineral composition of the culture medium, as well as the succession of different growth regulators, influenced the </span><i><span style="font-family:Verdana;">in vitro</span></i><span style="font-family:Verdana;"> growth of this species. Sterilized achenes of </span><i><span style="font-family:Verdana;">T. broussonetii</span></i><i> </i><span style="font-family:Verdana;">were able to germinate on an agar medium containing Gautheret macronutrients with a rate of 25% and a degree of contamination of less than 4%. Shoot apices of 15-day seedlings (two cotyledon leaves) were cultivated on SD + 0.46 μM Kin medium and the explants obtained were transplanted every month. Six macronutrients (MS, B</span>_{<span style="font-family:Verdana;">5</span>}<span style="font-family:Verdana;">, SH, SD, MS</span>_{<span style="font-family:Verdana;">m</span>} <span><span style="font-family:Verdana;">and N</span>_{<span style="font-family:Verdana;">30</span>}<span style="font-family:Verdana;">K) were tested and N</span>_{<span style="font-family:Verdana;">30</span>}<span style="font-family:Verdana;">K was chosen for the following experiments. Seven cytokinins (Kin, BAP, 2iP, DPU, adenine, Zeat and TDZ) at 0.46, 0.93 and 2.32 μM/l were evaluated and the addition of 0.93 μM adenine to N</span>_{<span style="font-family:Verdana;">30</span>}<span style="font-family:Verdana;">K medium favored significantly the induction of buds and the elongation of explants. Three polyamines (putrescine, spermidine and spermine) at 2, 5, 10 and 20 μM/l were tested. A better multiplication of buds, shoots and roots was noted