Analysis of PTEN Methylation Pattern by Pyrosequencing in Renal Cell Carcinoma

• 作者: Ademar Lopes Eurico Cleto Ribeiro de Campos Marcos Gonçalves Adriano Júnior Marcus Rivabem Winheski Renan Martins
• 来源期刊: 癌症治疗(英文)
• 学科: 医学
• 关键词: METHYLATION PTEN PYROSEQUENCING Renal Cell Carcinoma
• 年，卷（期）: azzlyw_2020,（12）
• 页码范围: 793-802
• 页数: 10页
• 分类号: R73

摘要

<strong>Objectives:</strong><span style="font-family:""><span style="font-family:Verdana;"> We aimed to determine the frequency of PTEN (phosphatase with tensin homology deleted in chromosome 10) hypermethylation in renal cell carcinoma (RCC) and its impact on overall (OS) and disease-free survival (DFS). The association between PTEN hypermethylation and clinic and pathologic factors was also analyzed. </span><b><span style="font-family:Verdana;">Materials and Methods: </span></b><span style="font-family:Verdana;">The authors analyzed 137 patients who had undergone a radical or partial nephrectomy between 1997 and 2011. The methylation pattern was quantified individually at multiple CpG islands that are located on the exon 1 of PTEN gene by pyrosequencing.</span></span><span style="font-family:""> </span><b><span style="font-family:Verdana;">Results:</span></b><span style="font-family:Verdana;"> Mean follow-up was 32</span><span style="font-family:Verdana;">.</span><span style="font-family:Verdana;">3 months. PTEN hypermethylation was detected with a low frequency (3.6%). There were no deaths or disease recurrence among patients with PTEN hypermethylation. Determining the association between PTEN hypermethylation and clinical and pathological factors was not possible due to the low frequency observed.</span><span style="font-family:""> </span><b><span style="font-family:Verdana;">Conclusion:</span></b><span style="font-family:Verdana;"> The results show that the methylation of promoter CpG sites in PTEN is relatively infrequent in renal carcinogenesis and has no prognostic impact on survival.</span>