Hepatoprotective Effects of the Leaves of Agauria salicifolia against Acetaminophen-Induced Liver Injury in Mice

• 作者: Agathe L. Fotio Christian K. Fofié Elvine P. Nguelefack-Mbuyo Lionel C. K. Bomgning Mathias K. Tsague Télesphore Benoît Nguelefack
• 来源期刊: 生物科学与医学(英文)
• 学科: 医学
• 关键词: Agauria salicifolia HEPATOPROTECTION ANTIOXIDANT HISTOLOGY
• 年，卷（期）: 2020,（6）
• 页码范围: 62-76
• 页数: 15页
• 分类号: R57

摘要

<em>Agauria</em><span><span><span> <i><span style="font-family:Verdana;">salicifolia</span></i><span style="font-family:Verdana;"> (Ericaceae) is a medicinal plant traditionally used for the treatment of liver ailments. The present study investigates the hepatoprotective effect of the residual aqueous fraction (RAF) of </span><i><span style="font-family:Verdana;">Agauria</span></i> <i><span style="font-family:Verdana;">salicifolia</span></i><span style="font-family:Verdana;"> on acetaminophen (APAP)-induced liver damage.</span><b> </b><span style="font-family:Verdana;">The ethanol extract obtained as maceration of the dried leaves, was fractionated into hexane, ethyl acetate and residual aqueous fractions. Adult mice of both sexes were pre-treated with the reference drug silymarin (50 mg/kg) or RAF (100 and 200 mg/kg) during 6 days followed by a single administration of APAP (500 mg/kg) on day 7. The hepatoprotective effect and the contribution of antioxidant activities were evaluated by determining the level of transaminases in serum samples, the levels of proteins, nitric oxide (NO), malondialdehyde (MDA), glutathione (GSH), superoxide dismutase (SOD), and catalase in liver homogenates. Histological analyses of liver slides were also performed. RAF at the doses of 100 and 200 mg/kg and silymarin significantly (</span><i><span style="font-family:Verdana;">p</span></i><span style="font-family:Verdana;"> < 0.001) decreased serum level of alanine and aspartate aminotransferases. RAF showed no effect on liver weight and its SOD content but significantly inhibited the</span><b> </b><span style="font-family:Verdana;">increase in proteins (</span><i><span style="font-family:Verdana;">p</span></i><span style="font-family:Verdana;"> < 0.01), NO (</span><i><span style="font-family:Verdana;">p</span></i><span style="font-family:Verdana;"> < 0.01) and MDA (</span><i><span style="font-family:Verdana;">p</span></i><span style="font-family:Verdana;"> < 0.001) induced by APAP while silymarin significantly reduced all these para