Phytochemical Characterization of Three Plants and Their Antisickling Activity in the Management of Sickle Cell Disease

• 作者: Antoinette Chiayé C. Yapo-Crezoit Ibrahime Sanogo Joel Akakpo-Akue Julien Koffi Golly Marcel Gnamien Ahon Mattieu Adou Kra Tatiana Kangah Mireille Kplé Yvette Fofie
• 来源期刊: 生物科学与医学(英文)
• 学科: 数学
• 关键词: Sickle Cell Disease Antisickling Activity Hydro-Ethanolic Extract J. gossypiifolia P. nigrescens J. secunda Côte d’Ivoire
• 年，卷（期）: 2020,（6）
• 页码范围: 100-112
• 页数: 13页
• 分类号: O17

摘要

<span style="line-height:1.5;">Natural plant products have been used by the population of the south-eastern part of C</span><span lang="EN-US" style="line-height:1.5;">&#244;</span><span style="line-height:1.5;">te d’Ivoire in the management of sickle cell anemia. </span><span style="line-height:1.5;">This study was aimed at investigating the antisickling activity of the hydro-ethanolic extract </span><span "="" style="line-height:1.5;"><span>of a combination of the leaves of </span><i><span>J</span></i><span>. </span><i><span>secunda</span></i><span>, </span><i><span>J</span></i><span>. </span><i><span>gossypiifolia</span></i></span><span style="line-height:1.5;"> a</span><span "="" style="line-height:1.5;"><span>nd </span><i><span>P</span></i><span>. </span><i><span>nigrescens</span></i><span>.</span></span><span style="line-height:1.5;"> These three plants species were used in the Ivorian traditional herbal medicine. Preliminary phytochemistry was carried out using standard methods. As for the sickling reversal test, the Hb SS blood sickling was induced with 2% sodium metabisulfite. After 120 minutes of incubation, the plant extract was added. Every 30 minutes for a period of 120 minutes, a drop of the prepared solution was observed at (40</span><span style="line-height:1.5;">×</span><span "="" style="line-height:1.5;"><span>) magnification and the percentage of reversion calculated. The phytochemical analysis revealed the presence of Alkaloids, Flavonoids, Polyphenols, Catechic Tannins, Sterols and Polyterpenes. The results of the reversal test showed that the percentage of sickling reversal effect of the combination of the plants (75.00 ± 4.33</span>^{<span>b,c</span>}<span>) was highly superior than the negative control (10.17 ± 0.55</span>^{<span>d</span>}<span>) but was significantly (p < 0.05) the same as the percentage of the individual plant (</span><i><span>J</span></i><span>. </span><i><span>secunda</span></i><span>: 83.50 ± 2.33</span>^{<span>a</span>}<span>;</span><i><