ERCC6L2 promotes DNA orientation-specific recombination in mammalian cells

• 作者: Bo O.Zhou Brian J.Lee Dingpeng Yang Fei-Long Meng Jiazhi Hu Junchao Dong Leng-Siew Yeap Liu Daisy Liu Min Huang Pengfei Dai Qiu Wu Rafael Casellas Shan Zha Tengfei Xiao Tingting Liu Wubing Zhang X.Shirley Liu Xiaojing Liu Xiaoqi Zheng Yafang Shang
• 来源期刊: 细胞研究（英文版）
• 年，卷（期）: 2020,（9）
• 所属期刊栏目: ARTICLES
• 页码范围: 732-744
• 页数: 13页
• 语种: 英文

摘要

Programmed DNA recombination in mammalian cells occurs predominantly in a directional manner.While random DNA breaks are typically repaired both by deletion and by inversion at approximately equal proportions,V(D)J and class switch recombination (CSR) of immunoglobulin heavy chain gene overwhelmingly delete intervening sequences to yield productive rearrangement.What factors channel chromatin breaks to deletional CSR in lymphocytes is unknown.Integrating CRISPR knockout and chemical perturbation screening we here identify the Snf2-family helicase-like ERCC6L2 as one such factor.We show that ERCC6L2 promotes double-strand break end-joining and facilitates optimal CSR in mice.At the cellular levels,ERCC6L2 rapidly engages in DNA repair through its C-terminal domains.Mechanistically,ERCC6L2 interacts with other end-joining factors and plays a functionally redundant role with the XLF end-joining factor in V(D)J recombination.Strikingly,ERCC6L2 controls orientation-specific joining of broken ends during CSR,which relies on its helicase activity.Thus,ERCC6L2 facilitates programmed recombination through directional repair of distant breaks.

作者信息

序号	姓名	单位	发文数	被引次数	H指数	G指数
1	Xiaojing Liu		1	0	0.0	0.0
2	Tingting Liu		1	0	0.0	0.0
3	Yafang Shang		1	0	0.0	0.0
4	Pengfei Dai		1	0	0.0	0.0
5	Wubing Zhang		1	0	0.0	0.0
6	Brian J.Lee		1	0	0.0	0.0
7	Min Huang		1	0	0.0	0.0
8	Dingpeng Yang		1	0	0.0	0.0
9	Qiu Wu		1	0	0.0	0.0
10	Liu Daisy Liu		1	0	0.0	0.0
11	Xiaoqi Zheng		1	0	0.0	0.0
12	Bo O.Zhou		1	0	0.0	0.0
13	Junchao Dong		1	0	0.0	0.0
14	Leng-Siew Yeap		1	0	0.0	0.0
15	Jiazhi Hu		1	0	0.0	0.0
16	Tengfei Xiao		1	0	0.0	0.0
17	Shan Zha		1	0	0.0	0.0
18	Rafael Casellas		1	0	0.0	0.0
19	X.Shirley Liu		1	0	0.0	0.0
20	Fei-Long Meng		1	0	0.0	0.0