Chang'an Ⅱ Decoction(肠安Ⅱ号方)-Containing Serum Ameliorates Tumor Necrosis Factor-α-Induced Intestinal Epithelial Barrier Dysfunction via MLCK-MLC Signaling Pathway in Rats

• 作者: BIAN Li-qun CHEN Ting JI Hai-jie KANG Nan LI Bao-shuang TANG Xu-dong WANG Feng-yun WANG Xiao-ge YIN Xiao-lan ZHANG Bei-hua ZHANG Yin-qiang
• 来源期刊: 中国结合医学杂志（英文版）
• 年，卷（期）: 2020,（10）
• 所属期刊栏目: Original Articles
• 页码范围: 745-753
• 页数: 9页
• 语种: 英文
• DOI: 10.1007/s11655-019-3034-6

摘要

Objective:To investigate the effect of Chang'an Ⅱ Decoction(肠安 Ⅱ 号方)-containing serum on intestinal epithelial barrier dysfunction in rats.Methods:Tumor necrosis factor (TNF)-α-induced injury of Caco-2 monolayers were established as an inflammatory model of human intestinal epithelium.Caco-2 monolayers were treated with blank serum and Chang'an Ⅱ Decoction-containing serum that obtained from the rats which were treated with distilled water and Chang'an Ⅱ Decoction intragastrically at doses of 0.49,0.98,1.96 g/(kg·d) for 1 week,respectively.After preparation of containing serum,cells were divided into the normal group,the model group,the Chang'an Ⅱ-H,M,and L groups (treated with 30 ng/mL TNF-α and medium plus 10％ high,middle-,and low-doses Chang'an Ⅱ serum,respectively).Epithelial barrier function was assessed by transepithelial electrical resistance (TER) and permeability of fluorescein isothiocyanate (FITC)-Iabeled dextran.Transmission electron microscopy was used to observe the ultrastructure of tight junctions (TJs).Immunofluorescence of zonula occludens-1 (ZO-1),claudin-1 and nuclear transcription factor-kappa p65 (NF-κ Bp65) were measured to determine the protein distribution.The mRNA expression of myosin light chain kinase (MLCK) was measured by real-time polymerase chain reaction.The expression levels of MLCK,myosin light chain (MLC) and p-MLC were determined by Westem blot.Results:Chang'an Ⅱ Decoction-containing serum significantly attenuated the TER and paracellular permeability induced by TNF-α.It alleviated TNF-α-induced morphological alterations in TJ proteins.The increases in MLCK mRNA and MLCK,MLC and p-MLC protein expressions induced by TNF-α were significantly inhibited in the Chang'an Ⅱ-H group.Additionally,Chang'an Ⅱ Decoction significantly attenuated translocation of NF-κ Bp65 into the nucleus.Conclusion:High-dose Chang'an Ⅱ-containing serum attenuates TNF-α-induced intestinal barrier dysfunction.The underlying mechanism may be involved in inhibiting the MLCK-MLC phosphorylation signaling pathway mediated by NF-κ Bp65.

作者信息

序号	姓名	单位	发文数	被引次数	H指数	G指数
1	CHEN Ting		1	0	0.0	0.0
2	YIN Xiao-lan		1	0	0.0	0.0
3	KANG Nan		1	0	0.0	0.0
4	WANG Xiao-ge		1	0	0.0	0.0
5	LI Bao-shuang		1	0	0.0	0.0
6	JI Hai-jie		1	0	0.0	0.0
7	ZHANG Yin-qiang		1	0	0.0	0.0
8	BIAN Li-qun		1	0	0.0	0.0
9	ZHANG Bei-hua		1	0	0.0	0.0
10	WANG Feng-yun		1	0	0.0	0.0
11	TANG Xu-dong		1	0	0.0	0.0