Severe <i>Citrus tristeza virus</i>Isolates from Eastern Mexico Are Related to the T36 Genotype Group

• 作者: Claudia Lomas-Barrié Cynthia G. Rodríguez-Quibrera Emiliano Loeza-Kuk Oscar Pérez-Hernández Patricia Rivas-Valencia Ricardo Santillán-Mendoza Santiago Domínguez-Monge
• 来源期刊: 美国植物学期刊(英文)
• 学科: 数学
• 关键词: CTV Epidemiology Severe Isolates Stem Pitting Quick Decline Coat Protein Gene
• 年，卷（期）: mgzwxqkyw_2020,（10）
• 页码范围: 1521-1532
• 页数: 12页
• 分类号: O17

摘要

<p style="text-align:justify;"> <span><i><span style="font-family:""><span style="font-family:Verdana;">Citrus </span><span style="font-family:Verdana;">tristeza</span><span style="font-family:Verdana;"> virus</span></span></i></span><span><span><span style="font-family:""><span style="font-family:Verdana;"> (CTV) outbreaks have been reported in the main citrus growing region of Mexico in the past four years. Recently, in eastern Mexico (the major citrus-growing region in the country), severe CTV isolates have been detected. However, the molecular identity of </span><span style="font-family:Verdana;">observed</span><span style="font-family:Verdana;"> isolates remains unestablished. This research was undertaken to elucidate the molecular characterization of CTV populations spreading in this region and to compare it with </span><span style="font-family:Verdana;">phylogeny</span><span style="font-family:Verdana;"> of existing isolates. Genotyping of 32 collected isolates was performed using reverse-transcription polymerase chain reaction (RT-PCR) with sequence analysis of the coat protein (CP) gene, putatively associated with pathogenicity. This protein is a 25 kDa major capsid protein, which forms a long virion body coating 95% of the particle length. </span><span style="font-family:Verdana;">A comparative</span><span style="font-family:Verdana;"> sequence </span><span style="font-family:Verdana;">analys</span></span></span></span><span style="font-family:Verdana;"><span style="font-family:Verdana;"><span style="font-family:Verdana;">is</span></span></span><span style="font-family:Verdana;"><span style="font-family:Verdana;"><span style="font-family:Verdana;"> was performed using CTV sequences from different geographical origins already published and deposited in the GenBank databases. Phylogenetic analysis showed that the degree of sequence divergence among isolates correlated with their pathogenicity. Based on the sequencing results, the collected isolates were categorizedn as mild or severe phylogenetic clusters