Detection of Adaptive Genetic Diversity in Wild Potato Populations and Its Implications in Conservation of Potato Germplasm

• 作者: John B. Bamberg
• 来源期刊: 美国植物学期刊(英文)
• 学科: 工学
• 关键词: Adaptive Genetic Diversity AFLP Markers Plant Population Structure Potato Germplasm Solanum fendleri
• 年，卷（期）: 2020,（10）
• 页码范围: 1562-1578
• 页数: 17页
• 分类号: TN9

摘要

A better understanding on how genetic diversity is structured at natural habitats can be helpful for exploration and acquisition of plant germplasm. Historically, studies have relied on DNA markers to elucidate potato genetic diversity. Current advances in genomics are broadening applications allowing the identification of markers linked to genomic regions under selection. Those markers, known as adaptive markers, unlock additional ways to value and organize germplasm diversity. For example, conservation priorities could be given to germplasm units containing markers associated to unique geographic identity, and/or linked to traits of tolerance to abiotic stresses. This study investigated if adaptive marker loci were possible to be identified in a large AFLP marker dataset of ninety-four populations of the wild potato species </span><i><span style="font-family:Verdana;">S. fendleri.</span></i><span style="font-family:Verdana;"> These populations originated from six different mountain ranges in southern Arizona, USA. A total of 2094 polymorphic AFLP markers were used to co</span><span style="font-family:Verdana;">nduct genetic diversity analyses of populations and mountain ranges. Adaptive markers were detected using Bayesian methods which distinguished marker loci departing significantly from frequencies expected under neutral models of genetic differentiation. This identified 16 AFLP loci that </span><span style="font-family:Verdana;">were considered to be adaptive. To contrast diversity p</span><span style="font-family:Verdana;">arameters generated with each set of markers, analyses that included all the 2094 AFLP markers, and only the 16 adaptive markers were conducted. The results showed that both were efficient for establishing genetic associations among populations and mountain ranges. However, adaptive markers were better on revealing geographic patterns and identity which would suggest these markers were linked to selection at the natural sites. An additional test to determine if adaptive markers associat