Comparative Analyses of the Efficacy of Dry and Fresh <i>Mangifera indica</i>Ethanolic Extracts on <i>E. coli</i>and <i>S. aureus</i>

• 作者: Monday Wisdom Nyimanu Omah
• 来源期刊: 生物科学与医学(英文)
• 学科: 医学
• 关键词: Mangifera indica Susceptibility Testing Herbal Drug Single and Combination Therapy
• 年，卷（期）: 2020,（9）
• 页码范围: 56-68
• 页数: 13页
• 分类号: R28

摘要

<strong>Background:</strong> The need to identify and characterize new antimicrobial agents is important due to the increasing development of resistance by microorganisms to the existing antimicrobial agents. <strong>Aim:</strong> This study examined the efficacies of <em>Mangifera indica</em> on <em>Escherichia coli</em> and <em>Staphylococcus aureus</em>. <strong>Method: </strong>Three parts (leaf [L], root [R], and bark [B]) of the plant were analyzed. The extraction of the samples was performed by aseptically grinding the samples, dissolving in absolute ethanol, and filtering through whatman filter paper. The efficacy of the extracts bothsingle and combined was determined using agar well diffusion assay with gentamycin [10 <em>μ</em>l] (<em>E. coli</em>) and vancomycin [30<em> μ</em>l] (<em>S. aureus</em>) as control antibiotics. <strong>Results: </strong>The higher concentration (C₂ = 3.0 g/ml) showed more antibacterial effectiveness than the lower concentration (C₁ = 1.5 g/ml) against both bacterial isolates with significant differences (<em>P</em> < 0.05) in all extracts except for single extracts (<em>E. coli</em> dry leaf extract;fresh bark extract), double extracts (<em>S. aureus</em>: dry and fresh leaf extracts) and triple extract (<em>E. coli </em>and <em>S. aureus</em> dry extracts). For the single extracts the bacteria has the following significant results: <em>E. coli</em> L (dry 6.3 ± 2.5 mm, fresh 14.7 ± 0.6 mm, <em>P</em> = 0.0050), R (dry 11.3 ± 1.5 mm, fresh 7.3 ± 1.5 mm, <em>P</em> = 0.0327);for<em> S. aureus</em> L (dry 7.0 ± 1.7 mm, fresh 11.0 ± 1.0 mm, <em>P</em> = 0.0257), R (dry 7.0 ± 2.0 mm, fresh 11.7 ± 1.5 mm, <em>P</em> = 0.0325), and B (dry 5.0 ± 1.0 mm, fresh 16.0 ± 1.0 mm, <em>P</em> = 0.0002). For the double extracts the bacteria has the following significant results: <em>E. coli </em>L + R (dry 15.7 ± 2.3 mm, fresh 1.7 ± 1.5 mm, <em>P</em> = 0.0070), R + B (dry 18.7 ± 1.5 mm, fresh 9.7 ± 1.5 mm, <em>P</em> = 0.0020), and L + B (dry 9.7 ± 1