RNAi Mediated Silencing of Gene Encoding 1-Deoxy-D-Xylulose-5-Phosphate Reductoisomerase (DXR) in <i>Centella asiatica</i>

• 作者: Basanta Kr. Borah Mahendra K. Modi Priyabrata Sen Ratna Kalita Richa Sharma
• 来源期刊: 美国植物学期刊(英文)
• 学科: 化学
• 关键词: Centella DXR RNAI Gene Knockdown Secondary Metabolite Agro Infiltration
• 年，卷（期）: mgzwxqkyw_2020,（11）
• 页码范围: 1723-1738
• 页数: 16页
• 分类号: O62

摘要

<i><span style="font-family:Verdana;">Centella asiatica </span></i><span style="font-family:""><span style="font-family:Verdana;">(L.) is one of the most valuable medicinal plants since preh</span><span style="font-family:Verdana;">istoric times. The pharmaceutical importance of this herb is due to the accumulation of large quantities of pentacyclic triterpenoid saponins, collectively known as centelloids synthesized by the isoprenoid biosynthesis</span><span style="font-family:Verdana;"> path</span><span style="font-family:Verdana;">way. Biosynthesis of triterpenoid in the plants proceeds via either of the tw</span><span style="font-family:Verdana;">o pathways, viz. Mevalonate (MVA) pathway (in the cytosol) or 2-C-methyl-D-erythritol 4-phosphate (MEP) pathway (in plastid). In </span><i><span style="font-family:Verdana;">Centella</span></i><span style="font-family:Verdana;">, the pathway leading to the accumulation of triterpenoid is still not known or elucidated. Thus, to know whether the MVA or MEP pathway or a cross-talk between the pathway leads to the biosynthesis of triterpenoid, silencing the key regulatory gene using RNAi tool, of each of the pathway and then analyze a metabolite is an efficient approach. The key regulatory enzyme of the MVA pathway </span><i><span style="font-family:Verdana;">i.e</span></i><span style="font-family:Verdana;">. 3-</span><i><span style="font-family:Verdana;">Hydroxy</span></i><span style="font-family:Verdana;">-3-</span><i><span style="font-family:Verdana;">methylglutaryl-coenzyme A reductase</span></i><span style="font-family:Verdana;"> (</span><i><span style="font-family:Verdana;">HMGR</span></i><span style="font-family:Verdana;">) has already been successfully silenced using RNAi tool</span></span><span style="font-family:Verdana;"> <a href="#ref1">[1]</a></span><span style="font-family:""><span style="font-family:Verdana;">. In the present study, the 1-</span><i><span style="font-family:Verdana;">deoxy-D-xylulose</span></i><span style="font-family:Verdana;">-5-</span><i