FHA2 is a plant-specific ISWI subunit responsible for stamen development and plant fertility

• 作者: and Xin-Jian He Bo-Wen Gu Cui-Jun Zhang Lian-Mei Tan She Chen Xiao-Mei Hou Xue-Wei Cai
• 来源期刊: 植物学报（英文版）
• 年，卷（期）: 2020,（11）
• 所属期刊栏目: Molecular Physiology
• 页码范围: 1703-1716
• 页数: 14页
• 语种: 英文
• DOI: 10.1111/jipb.12945

摘要

Imitation Switch (ISWI) chromatin remodelers are known to function in diverse multi-subunit complexes in yeast and animals. However, the constitution and function of ISWI complexes in Arabidopsis thaliana re-main unclear. In this study, we identified forkhead-associated domain 2 (FHA2) as a plant-specific subunit of an ISWI chromatin-remodeling complex in Arabidopsis. By in vivo and in vitro analyses, we demonstrated that FHA2 directly binds to RLT1 and RLT2, two redundant subunits of the ISWI complex in Arabidopsis. The stamen filament is shorter in the fha2 and rlt1/2 mutants than in the wild type, whereas their pistil lengths are com-parable. The shorter filament, which is due to reduced cell size, results in insufficient pollination and reduced fertility. The rlt1/2 mutant shows an early-flowering phenotype, whereas the phenotype is not shared by the fha2 mutant. Consistent with the functional specif-icity of FHA2, our RNA-seq analysis indicated that the fha2 mutant affects a subset of RLT1/2-regulated genes that does not include genes involved in the regulation of flowering time. This study demonstrates that FHA2 functions as a previously uncharacterized subunit of the Arabidopsis ISWI complex and is exclusively involved in regulating stamen development and plant fertility.

作者信息

序号	姓名	单位	发文数	被引次数	H指数	G指数
1	Bo-Wen Gu		1	0	0.0	0.0
2	Lian-Mei Tan		1	0	0.0	0.0
3	Cui-Jun Zhang		1	0	0.0	0.0
4	Xiao-Mei Hou		1	0	0.0	0.0
5	Xue-Wei Cai		1	0	0.0	0.0
6	She Chen		1	0	0.0	0.0
7	and Xin-Jian He		1	0	0.0	0.0