Dynamical and allosteric regulation of photoprotection in light harvesting complex Ⅱ

• 作者: Deyong Li Gianluigi Veglia Haisheng Ren Hao Li Heng Li Jiali Gao Luchao Du Manping Ye Mohan Wang Shanshan Li Yingjie Wang Yuxiang Weng
• 来源期刊: 中国科学:化学（英文版）
• 年，卷（期）: 2020,（8）
• 所属期刊栏目: ARTICLES
• 页码范围: 1121-1133
• 页数: 13页
• 语种: 英文

摘要

Major light-harvesting complex ofphotosystem Ⅱ (LHCⅡ) plays a dual role in light-harvesting and excited energy dissipation to protect photodamage from excess energy.The regulatory switch is induced by increased acidity,temperature or both.However,the molecular origin of the protein dynamics at the atomic level is still unknown.We carried out temperature-jump time-resolved infrared spectroscopy and molecular dynamics simulations to determine the energy quenching dynamics and conformational changes of LHCⅡ trimers.We found that the spontaneous formation of a pair of local α-helices from the 310-helix E/loop and the C-terminal coil of the neighboring monomer,in response to the increased environmental temperature and/or acidity,induces a scissoring motion of transmembrane helices A and B,shifting the conformational equilibrium to a more open state,with an increased angle between the associated carotenoids.The dynamical and allosteric conformation change leads to close contacts between carotenoid lutein 1 and chlorophyll pigment 612,facilitating the fluorescence quenching.Based on these results,we suggest a unified mechanism by which the LHCⅡ trimer controls the dissipation of excess excited energy in response to increased temperature and acidity,as an intrinsic result of intense sun light in plant photosynthesis.

作者信息

序号	姓名	单位	发文数	被引次数	H指数	G指数
1	Hao Li	中国科学院物理研究所	2	0	0.0	0.0
2	Yingjie Wang	中国科学院物理研究所	1	0	0.0	0.0
3	Manping Ye	中国科学院物理研究所	1	0	0.0	0.0
4	Shanshan Li	中国科学院物理研究所	1	0	0.0	0.0
5	Deyong Li	中国科学院物理研究所	1	0	0.0	0.0
6	Haisheng Ren	中国科学院物理研究所	1	0	0.0	0.0
7	Mohan Wang	中国科学院物理研究所	1	0	0.0	0.0
8	Luchao Du	中国科学院物理研究所	1	0	0.0	0.0
9	Heng Li	中国科学院物理研究所	1	0	0.0	0.0
10	Gianluigi Veglia	中国科学院物理研究所	1	0	0.0	0.0
11	Jiali Gao	中国科学院物理研究所	1	0	0.0	0.0
12	Yuxiang Weng	中国科学院物理研究所	1	0	0.0	0.0