Photodynamic Therapy with Phthalomethyl D: Perspectives against SARS-CoV-2

• 作者: Alexandre Gomes Sancho Aline Ronis Sampaio Carlos Henrique Machado Bittencourt Silva Esteban Fortuny Felice Picariello Lizia Fabiola Almeida Silva Marcus Vinicius de Mello Pinto Miriam Viviane Baron Rogério Mendonça de Carvalho
• 来源期刊: 生物科学与医学(英文)
• 学科: 医学
• 关键词: Photodynamic Therapy CORONAVIRUS Phthalocyanines Methylene Blue Vitamin D Phthalomethyl D
• 年，卷（期）: 2020,（10）
• 页码范围: 104-116
• 页数: 13页
• 分类号: R73

摘要

<strong>Background:</strong> Photodynamic therapy (PDT) is the generation of cytotoxic agents through the dynamic interaction between a photosensitizer excited by light at a specific wavelength. When associated with phthalocyanines, they are efficient in incorporating target cells and exhibiting high rates of triplet generation. This study aimed to characterize PDT associated with the phototherapeutic agent Phthalomethyl D, developed by the authors, in the process of repair, healing and immune improvement for possible application against SARS-CoV-2. <strong>Methods: </strong>Sixty-nine mice were used, divided into 2 groups: GI, treated with ILIB laser, without a phototherapeutic agent, and subjected to surgery for viral induction;GII, same as GI plus association of Phthalomethyl D. They were divided into subgroups and reevaluated at 7, 14, and 21 days, and then divided into 3 subgroups of 6 animals each, subjected to treatment at 24 h, 48 h, 72 h, 5 and 7 days. <strong>Results:</strong> Both groups had a high rate of partial incision closure and acute inflammatory control. Microscopically, there was a greater amount of amorphous fundamental substance, fibrocytes, fibroblasts, and giant cells and reduction in the number of keratinocytes, in the amount of keratin, and epidermal thickness in GII than in GI. <strong>Conclusions:</strong> PDT with Phthalomethyl D stimulates the processes of healing/repair and immunomodulation during viral infection, initially favoring the inflammatory response and, after 21 days, contributing to the anti-inflammatory response profile, making this approach possible in the treatment of individuals infected with SARS-CoV-2.