Regional Effect of APS-sEPS on Intestinal Structure and Mucosal Immunity in Mice

• 作者: Lei CHENG Liwei GUO Niandong YAN Qing JIN Rong CHEN Tao XIONG Wei ZHANG Xiaona ZHAO
• 来源期刊: 农业生物技术:英文版
• 学科: 医学
• 关键词: APS-sEPS MICE DIARRHEA Intestinal mucosa immunity Morphological structure
• 年，卷（期）: 2020,（6）
• 页码范围: 22-26
• 页数: 5页
• 分类号: R57

摘要

[Objectives]The aim of this study was to investigate the effects of APS-sEPS(a polysaccharide compound of Astragalus polysaccharides and sulfated Epimedium polysaccharide)on intestinal mucosal immunity and structural morphology.[Methods]Firstly,the diarrhea model was established using the optimal dose of magnesium sulfate in mice.Then,the diarrhea mice were randomly divided into three groups and given either physiological saline(diarrhea model group)or injected with APS-sEPS or APS.The normal mice were selected as a control group.After administration,the duodenum,jejunum and ileum were processed microtome section,and observed for describing the small intestine morphology,villus height and crypt depth.The tissue homogenates of the duodenum,jejunum and ileum were gathered to detect the changes of sIgA,IL-4 and IL-10.[Results]The results indicated that APS-sEPS could effectively relieve diarrhea in mice.In the APS-sEPS group,the villus heights of duodenum,jejunum and ileum were increased and the depth of crypt was reduced.The contents of IL-4,IL-10 and sIgA in jejunum and ileum in APS-sEPS group were significantly higher than that in the control group(P<0.05).[Conclusions]These results indicated that APS-sEPS promoted the recovery of intestinal morphological structure and enhanced the mucosa immunity of the small intestine.