Study on Virus-free and Rapid Propagation Technology of Artemisia selengensis sp.in Yangxin County

• 作者: Anhuai MING Jin ZHENG Rongxing YAN Shunwen XU Wei KANG Xianai GAO Yihe LI
• 来源期刊: 农业生物技术:英文版
• 学科: 农学
• 关键词: Artemisia selengensis sp. Culture in vitro Virus free Rapid propagation
• 年，卷（期）: 2020,（6）
• 页码范围: 70-72
• 页数: 3页
• 分类号: S51

摘要

[Objectives]This study was conducted to culture virus-free tissue culture plantlets of mid-maturing green-stalk Artemisia selengensis sp.varieties in Yangxin County.[Methods]With bud tips of A.selengensis in Yangxin as explants and MS as the basal medium,screening and proportioning of plant growth regulators were performed to establish a virus-free and rapid propagation system for mid-maturing green-stalk varieties of A.selengensis in Yangxin.[Results]The optimal callus induction medium,adventitious shoot differentiation medium,adventitious shoot elongation medium and rooting medium for explants were MS+6-BA 1.0 mg/L+NAA 0.5 mg/L+sucrose 25 g/L+agar 7 g/L,MS+6-BA 0.1 mg/L+NAA 0.1 mg/L+sucrose 25 g/L+agar 7 g/L,MS+6-BA 0.02 mg/L+NAA 0.02 mg/L+sucrose 25 g/L+agar 7 g/L and 1/2 MS+NAA 0.05 mg/L+sucrose 25 g/L+agar 7 g/L,respectively,and the seedling rate reached more than 95%.The culture conditions were as follows:temperature(25±2)℃,relative humidity 85%,illumination intensity 3000 lx,and illumination time 14 h/d.[Conclusions]This study has important practical significance for the purification and rejuvenation and large-scale industrial breeding of Yangxin A.selengensis seedlings.