Evaluation of Antibacterial Activities and Cytotoxicity of Three Medicinal Plants Used for the Management of <i>Mycobacterium tuberculosis</i>and <i>Staphylococcus aureus</i>Infections in the North-West Region of Cameroon

• 作者: Moses Samje Brice Tchoufack Paulin Ngoufo Henry M. Dilonga
• 来源期刊: 结核病研究(英文)
• 学科: 数学
• 关键词: ANTIBACTERIAL CYTOTOXICITY Medicinal Plants Mycobacterium tuberculosis Staphylococcus aureus
• 年，卷（期）: 2021,（1）
• 页码范围: 1-17
• 页数: 17页
• 分类号: O17

摘要

</span><b><span style="font-family:Verdana;">Introduction:</span></b><span style="font-family:Verdana;"></b> Chemotherapy is used to combat tuberculosis, and other microbial infections. Unfortunately, resistance has been reported to a vast majority of currently use drugs.</span></span><span style="font-family:""> </span><span style="font-family:Verdana;">The objective of this study was to search</span><span style="font-family:""><span style="font-family:Verdana;"> for new therapies from plant products for the sustainable management of tuberculosis and <i></span><i><span style="font-family:Verdana;">S. aureus</span></i><span style="font-family:Verdana;"></i></span><i> </i><span style="font-family:Verdana;">infections. <b></span><b><span style="font-family:Verdana;">Materials and Methods:</span></b><span style="font-family:Verdana;"></b> Crude extracts were obtained by sequential maceration of dry powdered plant material in three solvents;hexane, dichloromethane and methanol. Phytochemical screening to identify active constituents in the crude extracts was done by conventional methods. The antimycobacterial and antimicrobial activity on <i></span><i><span style="font-family:Verdana;">M. tuberculosis</span></i><span style="font-family:Verdana;"></i> control strain H37Ra and a clinical isolate of <i></span><i><span style="font-family:Verdana;">S. aureus</span></i><span style="font-family:Verdana;"></i> respectively, was evaluated at eight different concentrations using the microplatealamar blue assay. Cytotoxicity of the active extracts was evaluated on monkey kidney epithelial cells and assessed using the MTT/formazan assay. <b></span><b><span style="font-family:Verdana;">Results:</span></b><span style="font-family:Verdana;"></b> Twelve crude extracts were obtained, the hexane extract of <i></span><i><span style="font-family:Verdana;">Sanseviera</span></i></span><i><span style="font-family:""> </span></i><i><span style="font-family:Verdana;">liberica</span></i><span style="font-family:""><span style="font-family:Ver