Solid-State Fermentation Production of Chitosanase by <i>Streptomyces</i>with Waste Mycelia of <i>Aspergillus niger</i>

• 作者: Xiangyang Xu Zaiwei Song Yunchao Yin Faguo Zhong Junying Song Jiachao Huang Wangli Ye Peng Wang
• 来源期刊: 酶研究进展(英文)
• 学科: 经济
• 关键词: STREPTOMYCES Solid State Fermentation CHITOSANASE Waste Mycelia
• 年，卷（期）: 2021,（1）
• 页码范围: 10-18
• 页数: 9页
• 分类号: F42

摘要

Solid-state fermentation was carried out using mycelium powder of <em>Aspergillus niger</em> as substrate for the production of chitosanase of <em>Streptomyces</em>. Results of the experiments indicated that the optimal medium consisted of wheat bran and mycelium powder of <em>Aspergillus niger</em> with initial moisture content of 60% - 70%. The enzyme activity reached 41.33 U per gram dry medium after cultured for 5 days at 28<span style="white-space:nowrap;">&#176</span>C - 30<span style="white-space:nowrap;">&#176</span>C and an initial pH 6.5. Chitosanase was detected on the second day of incubation and had maximal activity at 5 days and decreased gradually within a 1 month period. Solid-state fermentation is maybe an economic alternative in the production.