Efficacy of a Biorevitalizing-Filler Solution on All Skin Aspects: 10 Years Approach through <i>in Vitro</i>Studies and Clinical Trials

• 作者: Sophie Robin Férial Fanian Carol Courderot-Masuyer Michel Tordjman Frederic Braccini Sylvie Boisnic Valérie Philippon Anne Grand Vincent Catherine Salomon Michel Manfait Philippe Humbert Philippe Piccerelle
• 来源期刊: 化妆品、皮肤病及应用期刊(英文)
• 学科: 工学
• 关键词: MESOTHERAPY Skin Rejuvenation NCTF 135HA® Hyaluronic Acid Skin Bio-revitalization Skin Booster
• 年，卷（期）: 2021,（1）
• 页码范围: 18-37
• 页数: 20页
• 分类号: TN9

摘要

<b>Introduction:</b> Skin aging is the result of many cellular dysfunctions over time particularly the fibroblasts and the keratinocytes. These dysfunctions could be decelerated by the preventive effects of some skin treatments such as Intradermal injections. NCTF 135HA has a polycomponent formulation designed to improve the efficacy of non-cross linked hyaluronic acid (as a micro-filler) on fibroblasts function. Although NCTF 135HA has been used by aesthetic practitioners since 20 years, we have analyzed all <i>in </i><i>vitro</i>, <i>ex vivo</i> and <i>in vivo</i> studies during past 10 years in order to summarized its anti-aging effect. <b>Methods</b><b>:</b> In these series of studies, the known effects of HA have been evaluated with this boosted formula. Collagen I synthesis, antiglycation effect and contractile forces developed by fibroblasts were studied. Fibroblasts and keratinocytes proliferations were evaluated in monolayer cells culture. The filling effect thanks to NCTF 135HA injection was <i>ex vivo </i>performed by fringe projection and the protective effect of NCTF 135HA against solar irradiation was performed <i>ex vivo</i> to study proliferation rate, elastin and collagen expression. Two clinical trials were performed on women to evaluate the anti-aging effect of NCTF 135HA injection. Hydration, firmness, radiance, wrinkles size, pore size, thickness and density of the dermis were analyzed. <b>Results</b><b>:</b> Collagen I, anti-AGE products, cell proliferation and contractile forces were significantly increased with NCTF 135HA in culture medium. <i>Ex vivo</i> studies showed that NCTF 135HA increased, cells proliferation rate, elastin and collagen production. In addition, the filling effect of the NCTF 135HA is significantly superior to placebo. The first clinical trial shows that NCTF 135HA injection significantly increased tonicity, hydration and radiance of the skin and significantly decreased wrinkle score. The second clinical trial demonstrated that NCTF 135HA injection significantly incre